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Anti-CLEC4F antibody

Cat.#: 175086

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Special Price 241.9 USD

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Product Information

  • Product Name
    Anti-CLEC4F antibody
  • Documents
  • Description
    Mouse monoclonal antibody to CLEC4F
  • Tested applications
    WB, FC
  • Species reactivity
    Human
  • Alternative names
    KCLR antibody; CLECSF13 antibody
  • Isotype
    Mouse IgG1
  • Preparation
    This antigen of this antibody was recombinant protein within human clec4f aa 90-500.
  • Clonality
    Monoclonal
  • Formulation
    Liquid, 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
  • Storage instructions
    Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
  • Applications

    WB:1:500-1:2,000

    FC:1:50-1:100

  • Validations

    Fig1:; Western blot analysis of CLEC4F on SH-SY5Y cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.

    Fig1:; Western blot analysis of CLEC4F on SH-SY5Y cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.

    Fig2:; Flow cytometric analysis of CLEC4F was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody ( 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Fig2:; Flow cytometric analysis of CLEC4F was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody ( 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

  • Background
    Q8N1N0
  • References
    • Yang CY. et. al. CLEC4F is an inducible C-type lectin in F4/80-positive cells and is involved in alpha-galactosylceramide presentation in liver. PLoS One. 2013 Jun 6;8(6):e65070.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"