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Anti-CKIIalpha (5F9) Mouse antibody

Cat.#: 168426

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Special Price 441.3 USD

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Product Information

  • Product Name
    Anti-CKIIalpha (5F9) Mouse antibody
  • Documents
  • Description
    CKIIalpha (5F9) Mouse monoclonal antibody
  • Tested applications
    WB, FC
  • Species reactivity
    Human, Mouse
  • Isotype
    Mouse IgG1
  • Preparation
    Antigen: Purified recombinant fragment of human CSNK2A2 (AA: 194-350) expressed in E. Coli.
  • Clonality
    Monoclonal
  • Formulation
    Ascitic fluid containing 0.03% sodium azide.
  • Storage instructions
    Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Applications

    WB: 1/500 - 1/2000

    FC: 1/200 - 1/400

    ELISA: 1/10000

  • Validations

    Western blot detection of CKII alpha prime polypeptide in C6,Jurkat,K562,3T3 and Hela cell lysates using CKII alpha prime polypeptide mouse mAb (1:2000 diluted).Predicted band size:41KDa.Observed band size:41KDa.

    Western blot detection of CKII alpha prime polypeptide in C6,Jurkat,K562,3T3 and Hela cell lysates using CKII alpha prime polypeptide mouse mAb (1:2000 diluted).Predicted band size:41KDa.Observed band size:41KDa.

    Flow cytometric analysis of HepG2 cells using CSNK2A2 mouse mAb (green) and negative control (red).

    Flow cytometric analysis of HepG2 cells using CSNK2A2 mouse mAb (green) and negative control (red).

    Western blot analysis using CSNK2A2 mouse mAb against HeLa (1), MCF-7 (2), HepG2 (3), Jurkat (4), NIH3T3 (5), and PC-12 (6) cell lysate.

    Western blot analysis using CSNK2A2 mouse mAb against HeLa (1), MCF-7 (2), HepG2 (3), Jurkat (4), NIH3T3 (5), and PC-12 (6) cell lysate.

  • Background
    Swiss-Prot Acc.P19784.Casein kinase II (CK2) is a constitutively active, ubiquitously expressed serine/threonine protein kinasethat is thought to have a regulatory function in cell proliferation, cell differentiation and apoptosis. CK2functions as a tetrameric complex consisting of two regulatory beta-subunits and two catalytic units (alphaand alpha') in a homomeric or heteromeric conformation. Whilst the alpha- and alpha'-subunits arecatalytically identical, proteins that regulate CK2, such as cdc2 and Hsp90, preferentially bind to thealpha and not the alpha'-subunit. CK2 can phosphorylate a number of key intracellular signaling proteinsimplicated in tumor suppression (p53 and PTEN) and tumorigenesis (myc, jun, NF-kappaB). CK2 is also thoughtto influence Wnt signaling via beta-catenin phosphorylation and the PI 3-K signaling pathway via thephosphorylation of Akt.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"