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Anti-CH25H antibody

Cat.#: 175775

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Special Price 241.9 USD

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Product Information

  • Product Name
    Anti-CH25H antibody
  • Documents
  • Description
    Rabbit polyclonal antibody to CH25H
  • Tested applications
    IHC-P, ICC/IF
  • Species reactivity
    Human
  • Alternative names
    C25H antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antigen of this antibody was synthetic peptide within human ch25h aa 181-272.
  • Clonality
    Polyclonal
  • Formulation
    Liquid, 1*TBS (pH7.4), 1%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.
  • Storage instructions
    Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
  • Applications

    IHC-P:1:100-500

    ICC/IF:1:100-500

  • Validations

    Fig1:; Immunohistochemical analysis of paraffin-embedded human brain glioma using anti-CH25H antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH; 2; O and PBS, and then probed with the primary antibody ( 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Fig1:; Immunohistochemical analysis of paraffin-embedded human brain glioma using anti-CH25H antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH; 2; O and PBS, and then probed with the primary antibody ( 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Background
    O95992
  • References
    • Lund E.G.et al.cDNA cloning of mouse and human cholesterol 25-hydroxylases, polytopic membrane proteins that synthesize a potent oxysterol regulator of lipid metabolism.J. Biol. Chem. 273:34316-34327(1998)

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