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Product Name
Anti-CEACAM1/CD66a antibody
- Documents
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Description
Rabbit polyclonal to CEACAM1/CD66a
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Tested applications
ELISA, IHC-P
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Species reactivity
Rat CEACAM1 / CD66a
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Alternative names
bb-1 antibody; Bgp antibody; BGP antibody; BGP1 antibody; Bgp1 antibody; BGPI antibody; Cc1 antibody; C-CAM antibody; CD66a antibody; CD66a antibody; Cea1 antibody; Cea-1 antibody; Cea7 antibody; Cea-7 antibody; Ceacam1 antibody; CEACAM1 antibody; Hv2 antibody; Hv-2 antibody; mCEA1 antibody; Mhv-1 antibody; MHVR antibody; MHVR1 antibody; mmCGM1 antibody; mmCGM1a antibody; mmCGM2 antibody; CC1 antibody; BGP antibody; BGP1 antibody; BGPI antibody; Bgp antibody; Hv2 antibody; Bgp1 antibody; Cea1 antibody; Cea7 antibody; Hv-2 antibody; MHVR antibody; bb-1 antibody; C-CAM antibody; CD66a antibody; Cea-1 antibody; Cea-7 antibody; MHVR1 antibody; Mhv-1 antibody; mCEA1 antibody; mmCGM1 antibody; mmCGM2 antibody; mmCGM1a antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Rat CEACAM1 / CD66a (rh CEACAM1 / CD66a; Met1-Ser422). CEACAM1 / CD66a specific IgG was purified by Rat CEACAM1 / CD66a affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Rat CEACAM1 / CD66a. The detection limit for Rat CEACAM1 / CD66a is < 0.039 ng/well.
IHC-P: 0.05-1 μg/mL
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Validations
CEACAM1 / CD66a Antibody, Rabbit PAb, Antigen Affinity Purified
Immunochemical staining of rat CEACAM1 in rat liver with rabbit polyclonal antibody (0.05 µg/mL, formalin-fixed paraffin embedded sections).
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Background
The carcinoembryonic-antigen-related cell-adhesion molecule (CEACAM) family of proteins has been implicated in various intercellular-adhesion and intracellular-signalling-mediated effects that govern the growth and differentiation of normal and cancerous cells. CEACAM1, also known as biliary glycoprotein I (BGP I) and CD66a, is a member of the carcinoembryonic antigen (CEA) gene family which belongs to the immunoglobulin superfamily. The highly glycosylated CEACAM1 contains one N-terminal V-type Ig-like domain and three C2-type Ig-like domains within its ECD, and one ITIM motif and a calmodulin binding site in the cytoplasmic region. CEACAM1 is a surface glycoprotein expressed on various blood cells, epithelial cells, and vascular cells. It was described as an adhesion molecule mediating cell adhesion via both homophilic and heterophilic manners, and was detected on leukocytes, epithelia, and endothelia. Studies have revealed that CEACAM1 performs actions in multiple cellular processes including tissue differentiation, angiogenesis, apoptosis, metastasis, as well as the modulation of innate and adaptive immune responses.
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References
- Gray-Owen SD, et al. (2006) CEACAM1: contact-dependent control of immunity. Nat Rev Immunol. 6(6): 433-46.
- Gu A, et al. (2009) Role of Ceacam1 in VEGF induced vasculogenesis of murine embryonic stem cell-derived embryoid bodies in 3D culture. Exp Cell Res. 315(10): 1668-82.
- Wong C, et al. (2009) CEACAM1 negatively regulates platelet-collagen interactions and thrombus growth in vitro and in vivo. Blood. 113(8): 1818-28.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"