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Product Name
Anti-CDNF/ARMETL1 antibody
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Description
Rabbit polyclonal to CDNF/ARMETL1
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Tested applications
ELISA, IHC-P
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Species reactivity
Mouse Acetylcholinesterase / ACHE
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Alternative names
9330140G23 antibody; RP24-129J15.1 antibody; Armetl1 antibody; ARMETL1 antibody; CDNF antibody; Cdnf antibody; MGC129430 antibody; ARMETL1 antibody; Armetl1 antibody; 9330140G23 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Mouse Acetylcholinesterase / ACHE (rM Acetylcholinesterase / ACHE; NP_033729.1; Met 1-Leu 614). Acetylcholinesterase / ACHE specific IgG was purified by Mouse Acetylcholinesterase / ACHE affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse ACHE. The detection limit for Mouse ACHE is approximately 0.00975 ng/well.
IHC-P: 0.1-2 μg/mL
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Validations
CDNF / ARMETL1 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of mouse ARMETL1 in mouse brain with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections).
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Background
Cerebral Dopamine Neurotrophic Factor (CDNF), also known as ARMETL1 (ARMET-like protein 1), is a secreted protein with eight conserved cysteine residues, predicting a unique protein fold and defining a new, evolutionarily conserved protein family. CDNF is a novel neurotrophic factor with strong trophic activity on dopaminergic neurons comparable to that of glial cell line-derived neurotrophic factor (GDNF). CDNF/ARMETL1 is a evolutionary conserved protein which can protect and restore the function of dopaminergic neurons in the rat model of Parkinson's disease, suggesting that CDNF might be beneficial for the treatment of Parkinson's disease. CDNF is widely expressed in neurons in several brain regions including cerebral cortex, hippocampus, substantia nigra, striatum and cerebellum. Human CDNF is glycosylated and secreted from transiently transfected cells. CDNF promotes the survival, growth, and function of dopamine-specific neurons and is expressed in brain regions that undergo cocaine-induced neuroplasticity.
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References
- Choi JM, et al. (2011) Analysis of mutations and the association between polymorphisms in the cerebral dopamine neurotrophic factor (CDNF) gene and Parkinson disease. Neurosci Lett. 493(3): 97-101.
- Sun ZP, et al. (2011) Intracellular trafficking and secretion of cerebral dopamine neurotrophic factor in neurosecretory cells. J Neurochem. 117(1): 121-32.
- Lohoff FW, et al. (2009) Association analysis between polymorphisms in the conserved dopamine neurotrophic factor (CDNF) gene and cocaine dependence. Neurosci Lett. 453(3): 199-203.
- Lindholm P, et al. (2007) Novel neurotrophic factor CDNF protects and rescues midbrain dopamine neurons in vivo. Nature. 448(7149): 73-7.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"