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Product Name
Anti-CD68 antibody
- Documents
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Description
Rabbit polyclonal to CD68
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Tested applications
ELISA, IHC-P
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Species reactivity
Rat CD68
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Alternative names
Cd68 antibody; CD68 antibody; DKFZp686M18236 antibody; gp110 antibody; GP110 antibody; macrosialin antibody; macrosialin antibody; Scard1 antibody; SCARD1 antibody; GP110 antibody; LAMP4 antibody; SCARD1 antibody; Lamp4 antibody; gp110 antibody; Scard1 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Rat CD68 (rh CD68; Q4FZY1; Met1-Ser295). CD68 specific IgG was purified by Rat CD68 affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
ELISA:0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Rat CD68. The detection limit for Rat CD68 is < 0.039 ng/well.
IHC-P: 0.1-2 μg/ml
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Validations
CD68 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
CD68 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
CD68 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
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Background
Macrosialin, also known as CD68 and Gp110, is a single-pass type I membrane protein which belongs to the LAMP family. CD68 is highly expressed by blood monocytes and tissue macrophages. It is also expressed in lymphocytes, fibroblasts and endothelial cells. CD68 is expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites. CD68 plays a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. It is a commonly used marker for macrophages. However, a number of studies have shown that CD68 antibodies react with other hematopoietic and non-hematopoietic cell types, suggesting that CD68 may not be a macrophage-specific antigen. CD68 binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.
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References
- Strobl H. et al., 1995, Br J Haematol. 90 (4): 774-82.
- Ogawa Y. et al., 1995, Pathol Int. 45 (9): 698-701.
- Sadovnikova E. et al., 2002,Leukemia. 16 (10): 2019-26.
- Gottfried E. et al., 2008, Scand J Immunol. 67 (5): 453-63.
- Strojnik T. et al., 2009, Anticancer Res. 29 (8): 3269-79.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"