Anti-CD63 (C-term) Rabbit antibody

Cat.#: 169125

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Product Information

  • Product Name
    Anti-CD63 (C-term) Rabbit antibody
  • Documents
  • Description
    CD63 (C-term) Rabbit polyclonal antibody
  • Tested applications
    WB, FC, IHC-P
  • Species reactivity
    Human
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: This CD63 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 163-190 amino acids from the C-terminal region of human CD63.
  • Clonality
    Polyclonal
  • Formulation
    Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
  • Applications

    WB::1:2000

  • Validations

    All lanes: Anti-CD63 Antibody (C-term) at 1:2000 dilutionnLane 1: A2058 whole cell lysatesnLane 2: A375 whole cell lysatesnLane 3: HL-60 whole cell lysatesnLane 4: THP-1 whole cell lysatesnLane 5: U-87 MG whole cell lysatesnnLysates/proteins at 20 u03bcg per lane. nnSecondarynGoat Anti-Rabbit IgG,  (H+L), Peroxidase conjugated at 1/10000 dilutionnnPredicted band size: 25 kDannBlocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Anti-CD63 Antibody (C-term) at 1:2000 dilutionnLane 1: A2058 whole cell lysatesnLane 2: A375 whole cell lysatesnLane 3: HL-60 whole cell lysatesnLane 4: THP-1 whole cell lysatesnLane 5: U-87 MG whole cell lysatesnnLysates/proteins at 20 u03bcg per lane. nnSecondarynGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionnnPredicted band size: 25 kDannBlocking/Dilution buffer: 5% NFDM/TBST.

    Overlay histogram showing HL-60 cells stained with 169125 (green line).  The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min.  The cells were then icubated in 2% bovine serum albumin to block non-spe

    Overlay histogram showing HL-60 cells stained with 169125 (green line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-spe

    AP5333B staining CD63 in human melanoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed,  paraffin-embedded sections).  Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval

    AP5333B staining CD63 in human melanoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval

    CD63 Antibody (C-term) (Cat. #169125) immunohistochemistry analysis in formalin fixed and paraffin embedded human skin carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.  This data demonstrates the use of the CD63 Antibody (C-term) for immunohistochemistry.  Clinical relevance has not been evaluated.

    CD63 Antibody (C-term) (Cat. #169125) immunohistochemistry analysis in formalin fixed and paraffin embedded human skin carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CD63 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

    All lanes: Anti-CD63 Antibody (C-term) at 1:1000 dilutionnLane 1: A2058 whole cell lysatesnLane 2: MDA-MB-453 whole cell lysatesnnLysates/proteins at 20 u03bcg per lane. nnSecondarynGoat Anti-Rabbit IgG,  (H+L), Peroxidase conjugated at 1/10000 dilutionnnPredicted band size: 26 kDannBlocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Anti-CD63 Antibody (C-term) at 1:1000 dilutionnLane 1: A2058 whole cell lysatesnLane 2: MDA-MB-453 whole cell lysatesnnLysates/proteins at 20 u03bcg per lane. nnSecondarynGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionnnPredicted band size: 26 kDannBlocking/Dilution buffer: 5% NFDM/TBST.

    Anti-CD63 Antibody (C-term)at  1:2000 dilution + human plasma lysatesnnLysates/proteins at 20 u03bcg per lane. nnSecondarynGoat Anti-Rabbit IgG,  (H+L), Peroxidase conjugated at 1/10000 dilutionnnPredicted band size: 25 kDannBlocking/Dilution buffer: 5% NFDM/TBST.

    Anti-CD63 Antibody (C-term)at 1:2000 dilution + human plasma lysatesnnLysates/proteins at 20 u03bcg per lane. nnSecondarynGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionnnPredicted band size: 25 kDannBlocking/Dilution buffer: 5% NFDM/TBST.

  • Background
    Swiss-Prot Acc.P08962.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"