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Product Name
Anti-CD46 antibody
- Documents
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Description
Mouse monoclonal to CD46
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Tested applications
FCM, IF, ICC/IF
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Species reactivity
Human CD46
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Alternative names
MCP antibody; AHUS2 antibody; CD46 antibody; Cd46 antibody; Mcp antibody; MGC26544 antibody; MIC10 antibody; TLX antibody; TRA2.10 antibody; MCP antibody; TLX antibody; AHUS2 antibody; MIC10 antibody; TRA2.10 antibody; Mcp antibody
- Immunogen
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Isotype
Mouse IgG1
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Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD46 (rh CD46; EAW93471.1; Met1-Asp328). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
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Clonality
Monoclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
FCM: 0.5-2 μg/Test
ICC/IF: 10-25 μg/mL
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Validations
CD46 Antibody, Mouse MAb, Immunofluorescence
Immunofluorescence staining of Human CD46 in Hela cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Mouse anti-Human CD46 monoclonal antibody (15 µg/ml) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 594-conjugated Goat Anti-mouse IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cell membrane.
CD46 Antibody, Mouse MAb, Flow cytometric analysis
Profile of anti-CD46 reactivity on HeLa cells analyzed by flow cytometry.
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Background
CD47 contains 1 Ig-like V-type (immunoglobulin-like) domain and is a receptor for the C-terminal cell binding domain of thrombospondin. It may play a role in membrane transport and signal transduction. CD47 is also a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. It is very broadly distributed on normal adult tissues, as well as ovarian tumors, being especially abundant in some epithelia and the brain. CD47 may play a role in membrane transport and/or integrin dependent signal transduction. It may prevent premature elimination of red blood cells. It also may be involved in membrane permeability changes induced following virus infection. By acting as an adhesion receptor for THBS1 on platelets, CD47 plays a role in both cell adhesion and in the modulation of integrins. It also plays an important role in memory formation and synaptic plasticity in the hippocampus.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"