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Product Name
Anti-CD40/TNFRSF5 antibody
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Description
Mouse monoclonal to CD40/TNFRSF5
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Tested applications
FCM
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Species reactivity
Rat CD40 / TNFRSF5
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Alternative names
p50 antibody; gp39 antibody; HIGM1 antibody; IGM antibody; IMD3 antibody; T-BAM antibody; TRAP antibody; p50 antibody; Bp50 antibody; CDW40 antibody; TNFRSF5 antibody; IGM antibody; p50 antibody; Bp50 antibody; GP39 antibody; IMD3 antibody; TRAP antibody; HIGM1 antibody; T-BAM antibody; Tnfrsf5 antibody; AI326936 antibody; AI326936 antibody; Bp50 antibody; Bp50 antibody; CD40 antibody; Cd40 antibody; CDW40 antibody; MGC9013 antibody; p50 antibody; RP23-428M13.1 antibody; TNFRSF5 antibody; Tnfrsf5 antibody
- Immunogen
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Isotype
Mouse IgG1
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Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Rat CD40 / TNFRSF5 (rR CD40 / TNFRSF5; Q4QQW2_RAT; Met1-Arg193) and conjugated with FITC under optimum conditions, the unreacted FITC was removed.
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Clonality
Monoclonal
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Formulation
Aqueous solution containing 0.5% BSA and 0.09% sodium azide
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Storage instructions
This antibody is stable for 12 months from date of receipt when stored at 2℃-8℃. Protected from prolonged exposure to light. Do not freeze !
Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal. -
Applications
FCM
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Validations
CD40 Antibody (FITC), Mouse MAb, Flow cytometric analysis
Flow cytometric analysis of Rat CD40 expression on rat splenocytes. SD splenocytes were stained with FITC-conjugated anti-Rat CD40 and PE conjugated anti-Rat CD3 (BD Pharmingen™ Cat. No. 550353). The dot plots were derived from events with the forward and side light-scatter characteristics of intact cells.
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Background
CD40, also known as TNFRSF5, is a member of the TNF receptor superfamily which are single transmembrane-spanning glycoproteins. CD40 protein plays an essential role in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. CD40 protein is expressed in B cells, dendritic cells, macrophages, endothelial cells, and several tumor cell lines. Defects in CD40 result in hyper-IgM immunodeficiency type 3 (HIGM3). In addition, CD40/CD40L interaction is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis.
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References
- van Kooten C, et al. (2000). CD40-CD40 ligand. J Leukoc Biol. 67 (1): 2-17.
- Bhushan A, et al. (2002). CD40:CD40L interactions in X-linked and non-X-linked hyper-IgM syndromes. Immunol Res. 24 (3): 311-24.
- Chatzigeorgiou A, et al. (2009) CD40/CD40L signaling and its implication in health and disease. Biofactors. 35(6): 474-83.
- Li R, et al. (2009) Expression of CD40 and CD40L in Gastric Cancer Tissue and Its Clinical Significance. Int J Mol Sci. 10(9): 3900-17.
- Lievens D, et al. (2009) The multi-functionality of CD40L and its receptor CD40 in atherosclerosis. Thromb Haemost. 102(2): 206-14.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"