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Product Name
Anti-CD297 antibody
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Description
Rabbit polyclonal to CD297
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Tested applications
ELISA, WB
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Species reactivity
Rat ART4
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Alternative names
DO antibody; DOK1 antibody; ARTC4 antibody; CD297 antibody; DO antibody; DOK1 antibody; ARTC4 antibody; 4432404K01Rik antibody; mono(ADP-ribosyl)transferase antibody; 4432404K01Rik antibody; ADP-ribosyltransferase 4 (DO blood group) antibody; CD297 antibody; DO antibody; DO antibody; DOK1 antibody; DOK1 antibody; Dombrock blood group carrier molecule antibody; ecto-ADP-ribosyltransferase 4 antibody; ecto-ADP-ribosyltransferase 4 antibody; mono(ADP-ribosyl)transferase 4 antibody; mono-ADP-ribosyltransferase 4 antibody; NAD(P)(+)--arginine ADP-ribosyltransferase 4 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Rat ART4 (rR ART4; F1MA10_RAT; Met1-Lys269). ART4 specific IgG was purified by Rat ART4 affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 10-20 μg/ml
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Rat ART4. The detection limit for Rat ART4 is < 0.039 ng/well.
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Validations
ART4 Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
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Background
ADP-ribosyltransferase 4 (Dombrock blood group), also known as Mono-ADP-ribosyltransferase 4(ART4), Dombrock blood group carrier molecule and CD297, is a protein that contains a mono-ADP-ribosylation (ART) motif. It is a member of the ADP-ribosyltransferase gene family but enzymatic activity has not been demonstrated experimentally. ADP-ribosyltransferase catalyzes the ADP-ribosylation of arginine residues in proteins. Mono-ADP-ribosylation is a posttranslational modification of proteins that is interfered with by a variety of bacterial toxins including cholera, pertussis, and heat-labile enterotoxins of E. coli. ART4 could be detected on HEL cells and erythrocytes by FACS analysis while it was absent from activated monocytes, despite the presence of ART4 mRNA in these cells. ART is also known as the carrier of the Dombrock blood group alloantigens (Do) which is glycosylphosphatidylinosotol-anchored to the erythrocyte membrane.
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References
- Parusel I, et al. (2005) A panel of monoclonal antibodies recognizing GPI-anchored ADP-ribosyltransferase ART4, the carrier of the Dombrock blood group antigens. Cell Immunol. 236(1-2): 59-65.
- Friedrich M, et al. (2005) Analysis of the 3' UTR of the ART3 and ART4 gene by 3' inverse RACE-PCR. DNA Seq. 16(1): 53-7.
- Okazaki IJ, et al. (1998) Glycosylphosphatidylinositol-anchored and secretory isoforms of mono-ADP-ribosyltransferases. J Biol Chem. 273(37): 23617-20.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"