-
Product Name
Anti-Cd1d1 antibody
- Documents
-
Description
Rabbit polyclonal antibody to Cd1d1
-
Tested applications
WB, FC
-
Species reactivity
Mouse, Rat
-
Alternative names
Cd1 antibody; Cd1a antibody; Cd1d antibody; Ly-3 antibody; CD1.1 antibody; Ly-38 antibody; AI747460 antibody
-
Isotype
Rabbit IgG
-
Preparation
This antigen of this antibody was klh conjugated synthetic peptide derived from mouse cd1d 165-270/336
-
Clonality
Polyclonal
-
Formulation
Liquid, 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
-
Storage instructions
Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
-
Applications
WB:1:500-2000
FC:1μg/Test
-
Validations
Fig1: Positive control: mouse thymocyte(2% Paraformaldehyde-fixed ); Isotype Control Antibody: Rabbit IgG; Dilution: 1μg in 100 μl 1 X PBS containing 0.5% BSA; Secondary Antibody: Goat anti-rabbit IgG-FITC; Dilution: 1:200 in 1 X PBS containing 0.5% BSA; Primary Antibody: Rabbit Anti-CD1A antibody ; Dilution: 1μg in 100 μl 1X PBS containing 0.5% BSA
Fig2: Blank control(mouse):mouse spleen cells (fixed with 2% paraformaldehyde (10 min), then permeabilized with 90% ice-cold methanol for 30 min on ice).; Primary Antibody: Rabbit Anti- CD1A antibody , Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );; Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Fig3: Sample:; Thymus (Mouse) Lysate at 40 ug; Primary: Anti-CD1d at 1/500 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 36 kD; Observed band size: 40 kD
- Background
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"