Anti-Cd1d1 antibody

Cat.#: 175314

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Product Information

  • Product Name
    Anti-Cd1d1 antibody
  • Documents
  • Description
    Rabbit polyclonal antibody to Cd1d1
  • Tested applications
    WB, FC
  • Species reactivity
    Mouse, Rat
  • Alternative names
    Cd1 antibody; Cd1a antibody; Cd1d antibody; Ly-3 antibody; CD1.1 antibody; Ly-38 antibody; AI747460 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antigen of this antibody was klh conjugated synthetic peptide derived from mouse cd1d 165-270/336
  • Clonality
    Polyclonal
  • Formulation
    Liquid, 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
  • Storage instructions
    Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
  • Applications

    WB:1:500-2000

    FC:1μg/Test

  • Validations

    Fig1: Positive control: mouse thymocyte(2% Paraformaldehyde-fixed ); Isotype Control Antibody: Rabbit IgG; Dilution: 1μg in 100 μl 1 X PBS containing 0.5% BSA; Secondary Antibody: Goat anti-rabbit IgG-FITC; Dilution: 1:200 in 1 X PBS containing 0.5% BSA; Primary Antibody: Rabbit Anti-CD1A antibody ; Dilution: 1μg in 100 μl 1X PBS containing 0.5% BSA

    Fig1: Positive control: mouse thymocyte(2% Paraformaldehyde-fixed ); Isotype Control Antibody: Rabbit IgG; Dilution: 1μg in 100 μl 1 X PBS containing 0.5% BSA; Secondary Antibody: Goat anti-rabbit IgG-FITC; Dilution: 1:200 in 1 X PBS containing 0.5% BSA; Primary Antibody: Rabbit Anti-CD1A antibody ; Dilution: 1μg in 100 μl 1X PBS containing 0.5% BSA

    Fig2: Blank control(mouse):mouse spleen cells (fixed with 2% paraformaldehyde (10 min), then permeabilized with 90% ice-cold methanol for 30 min on ice).; Primary Antibody: Rabbit Anti- CD1A antibody , Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );; Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.

    Fig2: Blank control(mouse):mouse spleen cells (fixed with 2% paraformaldehyde (10 min), then permeabilized with 90% ice-cold methanol for 30 min on ice).; Primary Antibody: Rabbit Anti- CD1A antibody , Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );; Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.

    Fig3: Sample:; Thymus (Mouse) Lysate at 40 ug; Primary: Anti-CD1d at 1/500 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 36 kD; Observed band size: 40 kD

    Fig3: Sample:; Thymus (Mouse) Lysate at 40 ug; Primary: Anti-CD1d at 1/500 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 36 kD; Observed band size: 40 kD

  • Background

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"