Anti-CD1C antibody

Cat.#: 175071

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Product Information

  • Product Name
    Anti-CD1C antibody
  • Documents
  • Description
    Mouse monoclonal antibody to CD1C
  • Tested applications
    WB, IHC-P, FC
  • Species reactivity
    Human
  • Alternative names
    R7 antibody; CD1 antibody; CD1A antibody; BDCA1 antibody
  • Isotype
    Mouse IgG2b
  • Preparation
    This antigen of this antibody was purified recombinant fragment of human cd1c (aa: extra 18-302) expressed in e. coli.
  • Clonality
    Monoclonal
  • Formulation
    Liquid, 1*PBS with 0.05% sodium azide.
  • Storage instructions
    Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
  • Applications

    WB: 1:500-1:2,000

    IHC-P: 1:50-1:200

    FC: 1:100-1:200

  • Validations

    Fig1: Western blot analysis of CD1C against human CD1C (AA: extra 18-302) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.

    Fig1: Western blot analysis of CD1C against human CD1C (AA: extra 18-302) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.

    Fig2: Western blot analysis of CD1C against HEK293 (1) and CD1C (AA: extra 18-302)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.

    Fig2: Western blot analysis of CD1C against HEK293 (1) and CD1C (AA: extra 18-302)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.

    Fig3: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using anti-CD1C antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody ( 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Fig3: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using anti-CD1C antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody ( 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Fig4: Flow cytometric analysis of CD1C was done on Ramos cells. The cells were fixed, permeabilized and stained with the primary antibody ( 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).

    Fig4: Flow cytometric analysis of CD1C was done on Ramos cells. The cells were fixed, permeabilized and stained with the primary antibody ( 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).

  • Background
  • References
    • Proc Natl Acad Sci U S A. 2016 Mar 1;113(9):E1266-75.
    • J Exp Med. 2014 Jun 30;211(7):1363-77.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"