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Product Name
Anti-CD16a/FCGR3A antibody
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Description
Rabbit polyclonal to CD16a/FCGR3A
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Tested applications
ELISA, WB, IP
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Species reactivity
Rat CD16a / FCGR3A
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Alternative names
RP11-5K23.1 antibody; CD16A antibody; IMD20 antibody; CD16 antibody; FCG3 antibody; CD16A antibody; FCGR3 antibody; IGFR3 antibody; IMD20 antibody; FCR-10 antibody; FCRIII antibody; FCGRIII antibody; FCRIIIA antibody; Fcrl3 antibody; CD16-2 antibody; FcgRIV antibody; Fcgr3a antibody; FcgammaRIV antibody; 4833442P21Rik antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Rat CD16a / FCGR3A (rR CD16a / FCGR3A; Met 1-Pro 200; ABO70343.1). CD16a / FCGR3A specific IgG was purified by Rat CD16a / FCGR3A affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 10-20 μg/mL
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Rat FCGR3A. The detection limit for Rat FCGR3A is approximately 0.00975 ng/well.
IP: 4-6 μg/mg of lysate
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Validations
CD16a / FCGR3A Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
CD16a / FCGR3A Antibody, Rabbit PAb, Antigen Affinity Purified, Immunoprecipitation
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Background
The Fc receptor with low affinity for IgG (FCGR3, or CD16) is encoded by 2 nearly identical genes, FCGR3A and FCGR3B, resulting in tissue-specific expression of alternative membrane-anchored isoforms. FCGR3A, it is also known as CD16a, encodes a transmembrane protein expressed on activated monocytes/macrophages, natural killer (NK) cells, and a subset of T cells.CD16a / FCGR3A is a receptor expressed on NK cells that facilitates antibody dependent cellular cytotoxicity (ADCC) by binding to the Fc portion of various antibodies. CD16a / FCGR3A also has a broader function. CD16a / FCGR3A is directly involved in the lysis of some virus-infected cells and tumor cells by NK cells, independent of antibody binding. Cross-linking of CD16a / FCGR3A on NK cells resulted in increased intracellular Ca2+ levels and a cascade of biochemical events similar to those activated by the T cell receptor. CD16a / FCGR3A on human NK cells is a lysis receptor that mediates the direct killing of some virus infected and tumor cells, independent of antibody ligation.
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References
- David Dornan, et al. Effect of FCGR2A and FCGR3A variants on CLL outcome. Blood. 2010 116: 4212-4222
- Edberg JC, et al. (2002) Genetic linkage and association of Fc gamma receptor IIIA (CD16A) on chromosome 1q23 with human systemic lupus erythematosus. Arthritis Rheum. 46(8): 2132-40.
- Li P, et al. (2002) Recombinant CD16A-Ig forms a homodimer and cross-blocks the ligand binding functions of neutrophil and monocyte Fcgamma receptors. Mol Immunol. 38(7): 527-38.
- Li P, et al. (2007) Affinity and kinetic analysis of Fcgamma receptor IIIa (CD16a) binding to IgG ligands. J Biol Chem. 282(9): 6210-21.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"