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Product Name
Anti-CD112/Nectin-2/PVRL2 antibody
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Description
Rabbit monoclonal to CD112/Nectin-2/PVRL2
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Tested applications
ELISA, WB, IP
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Species reactivity
Mouse PVRL2 / CD112 / Nectin-2
No cross-reactivity in ELISA with Human CD112 / Nectin-2 -
Alternative names
nectin-2 antibody; nectin-2 antibody; PRR2 antibody; PRR2 antibody; Pvrl2 antibody; PVRL2 antibody; PVRR2 antibody; HVEB antibody; PRR2 antibody; CD112 antibody; PVRR2 antibody; MPH antibody; Pvr antibody; Cd112 antibody; AI325026 antibody; AI987993 antibody; nectin-2 antibody; PVS antibody; Pvr antibody; PVS antibody; AI325026 antibody; AI987993 antibody; CD112 antibody; CD112 antibody; HVEB antibody; mHVEB antibody; MPH antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse PVRL2 extracellular domain (rM PVRL2; NP_033016.3; Met 1-Gly 351).
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Clonality
Monoclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 20-50 μg/ml
ELISA: 0.5-1 μg/mL
This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Mouse PVRL2. The detection limit for Mouse PVRL2 is 0.00245 ng/well.
IP: 1-4 μg/mg of lysate
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Validations
CD112 / Nectin-2 / PVRL2 Antibody, Rabbit MAb, Western blot
CD112 / Nectin-2 / PVRL2 Antibody, Rabbit MAb, Immunoprecipitation
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Background
Cluster of Differentiation 112 (CD112), also known as poliovirus receptor related protein 2 (PVRL2 or PRR2), is a single-pass type I transmembrane glycoprotein belonging to the Immunoglobulin superfamily. CD112 protein also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and thus is involved in cell to cell spreading of these viruses. CD112 protein has been identified as the ligand for DNAM-1 (CD226), and the interaction of CD226/CD112 protein can induce NK cell- and CD8+ T cell-mediated cytotoxicity and cytokine secretion. CD112 has been regarded as a critical component in allergic reactions, and accordingly may function as a novel target for anti-allergic therapy.
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References
- Bachelet I, et al. (2006) Mast cell costimulation by CD226/CD112 (DNAM-1/Nectin-2): a novel interface in the allergic process. J Biol Chem. 281(37): 27190-6.
- Wang L, et al. (2009) Molecular cloning, characterization and three-dimensional modeling of porcine nectin-2/CD112. Vet Immunol Immunopathol. 132(2-4): 257-63.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"