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Product Name
Anti-CD101 antibody
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Description
Rabbit polyclonal antibody to CD101
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Tested applications
WB, ICC/IF, FC
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Species reactivity
Human, Mouse, Rat
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Alternative names
V7 antibody; IGSF2 antibody; EWI-101 antibody
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Isotype
Rabbit IgG
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Preparation
This antigen of this antibody was klh conjugated synthetic peptide derived from human cd101 51-150/1021
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Clonality
Polyclonal
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Formulation
Liquid, 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
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Storage instructions
Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
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Applications
WB:1:500-2000
FC:1μg/Test
IF:1:10-100
ICC/IF:1:100-500
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Validations
Fig1: Protein: HepG2(human) lysates at 30ug;; Primary: rabbit Anti-CD101 at 1:300;; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000;; Predicted band size:113 kD Observed band size:113 kD
Fig2: Blank control(blue): U937(fixed with 2% paraformaldehyde (10 min)).; Primary Antibody:Rabbit Anti-CD101 antibody , Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );; Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
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