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Product Name
Anti-CCL2 antibody
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Description
Rabbit polyclonal antibody to CCL2
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Tested applications
WB
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Species reactivity
Mouse, Human
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Alternative names
HC11 antibody; MCAF antibody; MCP1 antibody; MCP-1 antibody; SCYA2 antibody; GDCF-2 antibody; SMC-CF antibody; HSMCR30 antibody
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Isotype
Rabbit IgG
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Preparation
This antigen of this antibody was synthetic peptide within human ccl2 aa 30-70 / 99.
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Clonality
Polyclonal
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Formulation
Liquid, 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
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Storage instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
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Applications
WB: 1:500-1:1,000
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Validations
Fig1:; Western blot analysis of CCL2 on mouse testis tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
- Background
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References
- Li Y.S. et. al. The expression of monocyte chemotactic protein (MCP-1) in human vascular endothelium in vitro and in vivo. Mol. Cell. Biochem. 126:61-68(1993).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"