Anti-CBL (6C5) Mouse antibody

Cat.#: 168353

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Product Information

  • Product Name
    Anti-CBL (6C5) Mouse antibody
  • Documents
  • Description
    CBL (6C5) Mouse monoclonal antibody
  • Tested applications
    WB, IHC-P, ICC/IF, FC, IP
  • Species reactivity
    Human, Mouse, Rat
  • Isotype
    Mouse IgG1
  • Preparation
    Antigen: Purified recombinant fragment of human C-CBL expressed in E. Coli.
  • Clonality
    Monoclonal
  • Formulation
    Ascitic fluid containing 0.03% sodium azide.
  • Storage instructions
    Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Applications

    WB: 1/500 - 1/2000

    IHC: 1/200 - 1/1000

    ICC: 1/200 - 1/1000

    FC: 1/200 - 1/400

    ELISA: 1/10000

  • Validations

    Western blot detection of c-Cbl in C6,Jurkat,K562,3T3 and Hela cell lysates using c-Cbl mouse mAb (1:1000 diluted).Predicted band size:120KDa.Observed band size:120KDa.

    Western blot detection of c-Cbl in C6,Jurkat,K562,3T3 and Hela cell lysates using c-Cbl mouse mAb (1:1000 diluted).Predicted band size:120KDa.Observed band size:120KDa.

    Immunoprecipitation analysis of Hela cell lysates using c-Cbl mouse mAb.

    Immunoprecipitation analysis of Hela cell lysates using c-Cbl mouse mAb.

    Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using C-CBL mouse mAb with DAB staining.

    Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using C-CBL mouse mAb with DAB staining.

    Flow cytometric analysis of MCF-7 cells using C-CBL mouse mAb (blue) and negative control (red).

    Flow cytometric analysis of MCF-7 cells using C-CBL mouse mAb (blue) and negative control (red).

    Immunofluorescence analysis of Hela cells using C-CBL mouse mAb (green). Blue

    Immunofluorescence analysis of Hela cells using C-CBL mouse mAb (green). Blue

    Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using C-CBL mouse mAb with DAB staining.

    Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using C-CBL mouse mAb with DAB staining.

  • Background
    Swiss-Prot Acc.P22681.The cbl oncogene was first identified as part of a transforming retrovirus which induces mouse pre-B and pro-B cell lymphomas. As an adaptor protein for receptor protein-tyrosine kinases, it positively regulates receptor protein-tyrosine kinase ubiquitination in a manner dependent upon its variant SH2 and RING finger domains. Ubiquitination of receptor protein-tyrosine kinases terminates signaling by marking active receptors for degradation.
  • References

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"