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Anti-cas9 antibody

Cat.#: 176702

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Special Price 193.5 USD

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Product Information

  • Product Name
    Anti-cas9 antibody
  • Documents
  • Description
    Rabbit polyclonal antibody to cas9
  • Tested applications
    WB, ELISA, ICC
  • Isotype
    IgG
  • Preparation
    This antigen of this antibody was recombinant protein
  • Clonality
    Polyclonal
  • Formulation
    Liquid, 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
  • Storage instructions
    Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
  • Applications

    WB: 1:2,000-1:5,000

    ELISA: 1:10,000

    ICC: 1:200-1:500

  • Validations

    Fig1: Western blot analysis on CRISPR-Cas9 transfected 293 cells (+) and non transtected 293 cells (-).

    Fig1: Western blot analysis on CRISPR-Cas9 transfected 293 cells (+) and non transtected 293 cells (-).

    Fig2: Immunofluorescence analysis of CRISPR-Cas9 transfected 293 cells. The cell expressing CRISPR-Cas9 can be detected using rabbit anti- CRISPR-Cas9 (1:500) followed by FITC (1:1000) goat anti-rabbit IgG.

    Fig2: Immunofluorescence analysis of CRISPR-Cas9 transfected 293 cells. The cell expressing CRISPR-Cas9 can be detected using rabbit anti- CRISPR-Cas9 (1:500) followed by FITC (1:1000) goat anti-rabbit IgG.

  • Background
    Q99ZW2
  • References
    • Genome-Scale CRISPR-Cas9 Knockout Screening in Human Cells. Shalem O, Sanjana NE, Hartenian E, Shi X, Scott DA, Mikkelson T, Heckl D, Ebert BL, Root DE, Doench JG, Zhang F. Science. 2013 Dec 12.
    • Multiplex Genome Engineering Using CRISPR/Cas Systems. Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini LA, Zhang F. Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub 2013 Oct 24.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"