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Product Name
Anti-Carboxypeptidase A2/CPA2 antibody
- Documents
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Description
Rabbit polyclonal to Carboxypeptidase A2/CPA2
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Tested applications
ELISA, WB
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Species reactivity
Rat Carboxypeptidase A2 / CPA2
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Alternative names
Carboxypeptidase A2 antibody; CPA2 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Rat Carboxypeptidase A2 / CPA2 (rR Carboxypeptidase A2 / CPA2; NP_001013101.2; Met1-Tyr417). Carboxypeptidase A2 / CPA2 specific IgG was purified by Rat Carboxypeptidase A2 / CPA2 affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 5-20 μg/ml
ELISA: 0.1-0.2 μg/ml
This antibody can be used at 0.1-0.2 μg/ml with the appropriate secondary reagents to detect Rat Carboxypeptidase A2 / CPA2.
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Validations
Carboxypeptidase A2 / CPA2 Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
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Background
Human Carboxypeptidase A2 ( CPA2 ) is a secreted pancreatic procarboxy -peptidase, and cleaves the C-terminal amide or ester bond of peptides that have a free C-terminal carboxyl group. The hydrolytic action of CPA2 was identified with a preference towards long substrates with aromatic amino acids in their C-terminal end, particularly tryptophan. CPA2 comprises a signal peptide, a pro region and a mature chain, and can be activated after cleavage of the pro peptide. Three different forms of human pancreatic procarboxypeptidase A have been isolated, and the A1 and A2 forms are always secreted as monomeric proteins with different biochemical properties.
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References
- Catasus, L. et al., 1995. J. Biol. Chem. 270: 6651-6657.
- Aloy, P. et al., 1998, Biol. Chem. 379: 149-155.
- Laethem, RM. et al., 1996, Arch. Biochem. Biophys.332: 8-18.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"