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Product Name
Anti-Carbonic Anhydrase XIV antibody
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Description
Rabbit monoclonal to Carbonic Anhydrase XIV
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Tested applications
ELISA, IHC-P, IF, ICC/IF
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Species reactivity
Human Carbonic Anhydrase XIV / CA14
No cross-reactivity in ELISA with Human CARPX; \ -
Alternative names
AW536446 antibody; CA14 antibody; Car14 antibody; Carbonic Anhydrase 14 antibody; Carbonic Anhydrase XIV antibody; Carbonic Anhydrase XIV antibody; CAXiV antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human Carbonic Anhydrase XIV / CA14 (rh Carbonic Anhydrase XIV / CA14; NP_036245.1; Met 1-Met 290).
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Clonality
Monoclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human CA14. The detection limit for Human CA14 is approximately 0.00245 ng/well.
IHC-P: 2-8 μg/mL
ICC/IF: 10-25 μg/mL
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Validations
Carbonic Anhydrase XIV / CA14 Antibody, Rabbit MAb, Immunohistochemistry
Immunochemical staining of human CA14 in human liver with rabbit monoclonal antibody (5 µg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to membrane of hepatocyte.
Carbonic Anhydrase XIV / CA14 Antibody, Rabbit MAb, Immunofluorescence
Immunofluorescence staining of Human CA14 in MCF7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-Human CA14 monoclonal antibody (15 µg/ml) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cell membrane.
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Background
The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. CAXIV is a member of CA family that showed an overall similarity of 29–46% to other active CA isozymes. The highest percentage similarity was with a transmembrane CA isoform, CAXII. The CAXIV was found high concentrations in human heart, brain, liver, and skeletal muscle but lower in the colon, small intestine, urinary bladder, and kidney. No CAXIV mRNA was seen in the salivary gland and pancreas. CAXIV is a likely candidate for the extracellular CA postulated to have an important role in modulating excitatory synaptic transmission in brain.
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References
- Lehtonen J, et al. (2004) Characterization of CA XIII, a Novel Member of the Carbonic Anhydrase Isozyme Family. The Journal of Biological Chemistry. 279: 2719-27.
- Lindskog S. (1997) Structure and mechanism of carbonic anhydrase. Pharmacology & Therapeutics. 74(1)：1-20.
- Parkkila S, et al. (2001) Expression of membrane-associated carbonic anhydrase XIV on neurons and axons in mouse and human brain. PNAS. 98(4): 1918-23.
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