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Product Name
Anti-Carbonic Anhydrase XII antibody
- Documents
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Description
Rabbit polyclonal to Carbonic Anhydrase XII
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Tested applications
ELISA, IHC-P
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Species reactivity
Mouse Ca12 / Car12
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Alternative names
2310047E01Rik antibody; AI314958 antibody; CA12 antibody; Car12 antibody; Carbonic Anhydrase 12 antibody; Carbonic Anhydrase XII antibody; Carbonic Anhydrase XII antibody; CAXII antibody; FLJ20151 antibody; HsT18816 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Mouse Ca12 (rM Ca12; NP_848483.2; Met 1-Ser 301). Ca12 specific IgG was purified by mouse Ca12 affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
ELISA: 0.5-1 μg/mL
This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Mouse Ca12. The detection limit for Mouse Ca12 is 0.00245 ng/well.
IHC-P: 0.1-2 μg/mL
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Validations
Carbonic Anhydrase XII / Car12 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of mouse CA12 in mouse kidney with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to nephric tubule.
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Background
Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes first discovered in 1933 that catalyze the reversible hydration of carbon dioxide. CAs participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. CA12, also known as Car12 and carbonic anhydrase XII, is a type I membrane enzyme of an N-terminal extracellular catalytic domain, a membrane-spanning α-helix, and a small intracellular C-terminal domain. It is highly expressed in colon, kidney, prostate, intestine and activated lymphocytes and moderately expressed in pancreas, ovary, and testis. Overexpression of the CA12 is observed in certain human cancers and is used as a tumor marker. rmCA12 corresponds to the extracellular domain and has both carbonic anhydrase activity and esterase activity.
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References
- Sahin, U. et al., 1996, Proc. Natl. Acad. Sci. U.S.A. 92 (25): 11810–11813.
- Ivanov, S.V. et al., 1998, Proc. Natl. Acad. Sci. USA 95:12596 - 12601.
- Strausberg, R.L. et al., 2002, Proc. Natl. Acad. Sci. USA 99:16899 - 16903.
- Liao, S.Y. et al., 2003, J. Med. Genet. 40:257 - 262.
- Supuran, C. T. et al., 2008, Curr Pharm Des. 14 (7): 601-602.
- Elleuche, S. et al., 2009, Curr Genet. 55 (2): 211-222.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"