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Product Name
Anti-Carbonic Anhydrase VIII/CA8 antibody
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Description
Rabbit polyclonal to Carbonic Anhydrase VIII/CA8
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Tested applications
ELISA, WB, IHC-P, IP
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Species reactivity
Mouse Carbonic Anhydrase VIII / CA8
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Alternative names
AW546993 antibody; Ca8 antibody; CA8 antibody; CALS antibody; Cals antibody; Cals1 antibody; CAMRQ3 antibody; Car8 antibody; Carbonic Anhydrase VIII antibody; Carbonic Anhydrase VIII antibody; CARP antibody; Carp antibody; CA-VIII antibody; MGC120502 antibody; MGC99509 antibody; RP23-180H12.1 antibody; wdl antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Mouse Carbonic Anhydrase VIII / CA8 (rh Carbonic Anhydrase VIII / CA8; P28651; Met1-Gln291). Carbonic Anhydrase VIII / CA8 specific IgG was purified by Mouse Carbonic Anhydrase VIII / CA8 affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 10-20 μg/mL
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse Carbonic Anhydrase VIII / CA8. The detection limit for Mouse Carbonic Anhydrase VIII / CA8 is < 0.039 ng/well.
IHC-P: 0.1-1 μg/mL
IP: 1-4 μg/mg of lysate
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Validations
Carbonic Anhydrase VIII / CA8 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunoprecipitation
Carbonic Anhydrase VIII / CA8 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of mouse CA8 in mouse brain with rabbit polyclonal antibody (0.2 µg/mL, formalin-fixed paraffin embedded sections).
Carbonic Anhydrase VIII / CA8 Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
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Background
The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase protein (CA) VIII, which is a member of the CA gene family, has been shown to have no catalytic CA activity and its biological function is still unknown. Increased expression of CA-RP VIII was observed in 78% of colorectal carcinomas. It suggested that CA-RP VIII plays a role in the process of invasion in colorectal cancer.
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References
- Lehtonen J, et al. (2004) Characterization of CA XIII, a Novel Member of the Carbonic Anhydrase Isozyme Family. The Journal of Biological Chemistry. 279: 2719-27.
- Lindskog S. (1997) Structure and mechanism of carbonic anhydrase. Pharmacology & Therapeutics. 74(1)：1-20.
- Miyaji E, et al. (2003) Overexpression of carbonic anhydrase-related protein VIII in human colorectal cancer. The Journal of Pathology. 201(1): 37-45.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"