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Product Name
Anti-Carbonic Anhydrase VA antibody
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Description
Rabbit polyclonal to Carbonic Anhydrase VA
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Tested applications
ELISA, WB
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Species reactivity
Human Carbonic Anhydrase VA / CA5A
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Alternative names
Ca5a antibody; CA5A antibody; CAV antibody; CA-VA antibody; CA-VA antibody; CAVA antibody; CA Y antibody; CA5 antibody; Car5 antibody; Car5a antibody; Carbonic Anhydrase VA antibody; Carbonic Anhydrase VA antibody; CAV antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Human Carbonic Anhydrase VA / CA5A (rh Carbonic Anhydrase VA / CA5A; NP_001730.1; Ala 40-Ser 305). Carbonic Anhydrase VA / CA5A specific IgG was purified by Human Carbonic Anhydrase VA / CA5A affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 5-10 μg/mL
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human CA5A. The detection limit for Human CA5A is approximately 0.00245 ng/well.
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Validations
Carbonic Anhydrase VA / CA5A Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
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Background
Carbonic anhydrase 5A, mitochondrial, also known as Carbonate dehydratase VA, Carbonic anhydrase VA, CA-VA and CA5A, is a member of the alpha-carbonic anhydrase family. Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes first discovered in 1933 that catalyze the reversible hydration of carbon dioxide. CAs participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. CA5A / CA-VA is activated by histamine, L-adrenaline, L- and D-histidine, and L- and D-phenylalanine. It is inhibited by coumarins, sulfonamide derivatives such as acetazolamide and Foscarnet (phosphonoformate trisodium salt).
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References
- Strausberg, R.L. et al., 2002, Proc. Natl. Acad. Sci. USA 99:16899 - 903.
- Liao, S.Y. et al., 2003, J. Med. Genet. 40:257 - 262.
- Temperini C.et al., 2006, Chemistry 12: 7057-66.
- Temperini C.et al., 2006, J. Med. Chem. 49: 3019-27.
- Supuran, C. T. et al., 2008, Curr Pharm Des. 14 (7): 601-602.
- Elleuche, S. et al., 2009, Curr Genet. 55 (2): 211-222.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"