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Product Name
Anti-Carbonic Anhydrase IV / Car4 / CA4 antibody
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Description
Rabbit monoclonal to Carbonic Anhydrase IV / Car4 / CA4
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Tested applications
ELISA, IHC-P
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Species reactivity
Human Carbonic Anhydrase IV / CA4
No cross-reactivity in ELISA with Human CA2; Human CA3; Human CA5A; Human CA5B; Human CA8; Human CA9; Human CA10; Human CA12; Human CA13; Human CA14 -
Alternative names
Ca4 antibody; CA-IV antibody; Car4 antibody; Carbonic Anhydrase IV antibody; Carbonic Anhydrase IV antibody; CAIV antibody; Car4 antibody; RP17 antibody; RP23-167D6.1 antibody; AW456718 antibody; CA IV antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human Carbonic Anhydrase IV / CA4 (rh Carbonic Anhydrase IV / CA4; NP_000708.1; Met1-Lys283).
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Clonality
Monoclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human Carbonic Anhydrase 4 / CA4. The detection limit for Human Carbonic Anhydrase 4 / CA4 is approximately 0.039 ng/well.
IHC-P: 1-10 μg/mL
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Validations
Carbonic Anhydrase IV / CA4 Antibody, Rabbit MAb, Immunohistochemistry
Immunochemical staining of human CA4 in human kidney with rabbit monoclonal antibody (5 µg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to renal tubules.
Carbonic Anhydrase IV / CA4 Antibody, Rabbit MAb, Immunohistochemistry
Immunochemical staining of human CA4 in human lung with rabbit monoclonal antibody (5 µg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to pulmonary capillaries.
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Background
The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase IV (CAIV) is a membrane-associated enzyme anchored to plasma membrane surfaces by a phosphatidylinositol glycan linkage. CAIV is a high-activity isozyme in CO2 hydration comparable to that of CAII. Furthermore, CAIV is more active in HCO3- dehydration than is CAII. However, the esterase activity of CAIV is decreased 150-fold compared to CAII.
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References
- Lehtonen J, et al. (2004) Characterization of CA XIII, a Novel Member of the Carbonic Anhydrase Isozyme Family. The Journal of Biological Chemistry. 279: 2719-27.
- Lindskog S. (1997) Structure and mechanism of carbonic anhydrase. Pharmacology & Therapeutics. 74(1)：1-20.
- Baird TT, et al. (1997) Catalysis and Inhibition of Human Carbonic Anhydrase IV. Biochemistry. 36 (9): 2669-78.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"