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Product Name
Anti-Carbonic Anhydrase II antibody
- Documents
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Description
Rabbit polyclonal to Carbonic Anhydrase II
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Tested applications
ELISA, WB, IHC-P, IP
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Species reactivity
Mouse Carbonic Anhydrase II / CA2
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Alternative names
AI131712 antibody; CA2 antibody; Ca2 antibody; CAII antibody; CAII antibody; CA-II antibody; Car2 antibody; Car-2 antibody; Car2 antibody; Carbonic Anhydrase II antibody; Carbonic Anhydrase II antibody; Ltw-5 antibody; Lvtw-5 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Mouse Carbonic Anhydrase II / CA2 (rM Carbonic Anhydrase II / CA2; AAH55291.1; Met 1-Lys 260). Carbonic Anhydrase II / CA2 specific IgG was purified by Mouse Carbonic Anhydrase II / CA2 affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 2-10 μg/mL
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse CA2. The detection limit for Mouse CA2 is approximately 0.00975 ng/well.
IHC-P: 0.1-2 μg/mL
IP: 4-6 μg/mg of lysate
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Validations
Carbonic Anhydrase II / Car2 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of mouse CA2 in mouse kidney with rabbit polyclonal antibody (0.2 µg/mL, formalin-fixed paraffin embedded sections).
Carbonic Anhydrase II / Car2 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of mouse CA2 in mouse stomach with rabbit polyclonal antibody (0.2 µg/mL, formalin-fixed paraffin embedded sections).
Carbonic Anhydrase II / Car2 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of mouse CA2 in mouse intestine with rabbit polyclonal antibody (0.2 µg/mL, formalin-fixed paraffin embedded sections).
Carbonic Anhydrase II / Car2 Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
Carbonic Anhydrase II / Car2 Antibody, Rabbit PAb, Antigen Affinity Purified
Carbonic Anhydrase II / Car2 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of mouse CA2 in mouse brain with rabbit polyclonal antibody (0.2 µg/mL, formalin-fixed paraffin embedded sections).
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Background
The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase II is one of fourteen forms of human α carbonic anhydrases. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Renal carbonic anhydrase allows the reabsorption of sodium ions in the proximal tubule. Carbonic anhydrase II has been shown to interact with Band 3 and Sodium-hydrogen antiporter 1.
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References
- Lehtonen J, et al. (2004) Characterization of CA XIII, a Novel Member of the Carbonic Anhydrase Isozyme Family. The Journal of Biological Chemistry. 279: 2719-27.
- Lindskog S. (1997) Structure and mechanism of carbonic anhydrase. Pharmacology & Therapeutics. 74(1)：1-20．
- Lilias A, et al. (1972) Crystal Structure of Human Carbonic Anhydrase C. Nature new biology. 235: 131-7.
- Li XJ, et al. (2002) Carbonic Anhydrase II Binds to and Enhances Activity of the Na+/H+ Exchanger. The Journal of Biological Chemistry. 277: 36085-91.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"