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Product Name
Anti-Calumenin antibody
- Documents
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Description
Rabbit polyclonal to Calumenin
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Tested applications
ELISA, WB, IP
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Species reactivity
Human Calumenin / CALU
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Alternative names
9530075H20Rik antibody; Calu antibody; CALU antibody; Calumenin antibody; Calumenin antibody; crocalbin antibody; crocalbin antibody; crocalbin-like protein antibody; IEF SSP 9302 antibody; multiple EF-hand protein antibody; 9530075H20Rik antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Human Calumenin / CALU (rh Calumenin / CALU; O43852-1; Met1-Arg311). Calumenin / CALU specific IgG was purified by Human Calumenin / CALU affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 2-10 μg/ml
ELISA: 0.1-0.2 μg/ml
This antibody can be used at 0.1-0.2 μg/ml with the appropriate secondary reagents to detect Human Calumenin / CALU. The detection limit for Human Calumenin / CALU is < 0.039 ng/well.
IP: 4-6 uL/mg of lysate
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Validations
Calumenin / CALU Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
Calumenin / CALU Antibody, Rabbit PAb, Antigen Affinity Purified, Immunoprecipitation
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Background
Calumenin belongs to the CREC family. It contains 6 EF-hand domains. Calumenin is expressed in skeletal muscle (at protein level). Calumenin interacts with GGCX and RYR1 in the presence of calcium ions, but not in the presence of EDTA. Calumenin is Involved in regulation of vitamin K-dependent carboxylation of multiple N-terminal glutamate residues. It seems to inhibit gamma-carboxylase GGCX. Calumenin also binds 7 calcium ions with a low affinity and may modulate calcium release from the sarcoplasmic reticulum.
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References
- Hartley JL, et al. (2001) DNA cloning using in vitro site-specific recombination. Genome Res. 10 (11):1788-95.
- Vorum H, et al. (2000) Calumenin interacts with serum amyloid P component. FEBS Lett. 465 (2-3):129-34.
- Vorum H, et al. (1999) n calumenin localizes to the secretory pathway and is secreted to the medium. Exp Cell Res. 248(2):473-81.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"