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Product Name
Anti-Calreticulin antibody
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Description
Rabbit polyclonal to Calreticulin
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Tested applications
ELISA, WB, IHC-P, IP
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Species reactivity
Human Calreticulin
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Alternative names
Calr antibody; CALR antibody; Calregulin antibody; calregulin antibody; Calreticulin antibody; Calreticulin antibody; calreticulin) antibody; cC1qR antibody; CRP55 antibody; CRP55 antibody; CRT antibody; CRT antibody; endoplasmic reticulum resident protein 60 antibody; endoplasmic reticulum resident protein 60 antibody; ERp60 antibody; ERp60 antibody; grp60 antibody; HACBP antibody; HACBP antibody; RO antibody; Sicca syndrome antigen A (autoantigen Ro antibody; SSA antibody; RO antibody; CRT antibody; SSA antibody; cC1qR antibody; HEL-S-99n antibody; CRT antibody; Calregulin antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Human Calreticulin (rh Calreticulin; P27797; Met1-Ala413). Calreticulin specific IgG was purified by Human Calreticulin affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 2-10 μg/ml
ELISA:0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human Calreticulin. The detection limit for Human Calreticulin is < 0.039 ng/well.
IHC-P: 0.05-1 μg/ml
IP: 1-4 μg/mg of lysate
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Validations
Calreticulin Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
Calreticulin Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Calreticulin Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Calreticulin Antibody, Rabbit PAb, Antigen Affinity Purified, Immunoprecipitation
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Background
Calreticulin is a multifunctional protein. It acts as a main Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. Calreticulin binds Ca2+ ions (a second messenger in signal transduction), rendering it inactive. The Ca2+ is bound with low affinity, but high capacity, and can be released on a signal. Located in storage compartments associated with the endoplasmic reticulum, calreticulin also binds to misfolded proteins and prevents them from being exported from the endoplasmic reticulum to the golgi apparatus. The amino terminus of calreticulin interacts with the DNA-binding domain of the glucocorticoid receptor and prevents the receptor from binding to its specific glucocorticoid response element. Calreticulin reduces the binding of androgen receptor to its hormone-responsive DNA element and inhibits androgen receptor and retinoic acid receptor transcriptional activities in vivo, as well as retinoic acid-induced neuronal differentiation. Therefore, calreticulin acts as a significant modulator of the regulation of gene transcription by nuclear hormone receptors.
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References
- Michalak M, et al. (2002) Calreticulin in cardiac development and pathology. Biochim Biophys Acta. 1600(1-2):32-7.
- Chao MP, et al. (2010) Calreticulin is the dominant pro-phagocytic signal on multiple human cancers and is counterbalanced by CD47. Sci Transl Med. 2(63):63ra94.
- Andrin, C, et al. (1998) Interaction between a Ca2+-binding protein calreticulin and perforin, a component of the cytotoxic T-cell granules. Biochemistry. 37(29):10386-94.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"