Anti-CACNG3 antibody

Cat.#: 175129

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Product Information

  • Product Name
    Anti-CACNG3 antibody
  • Documents
  • Description
    Rabbit polyclonal antibody to CACNG3
  • Tested applications
    WB, ICC, IHC-P, FC
  • Species reactivity
    Human, Rat
  • Isotype
    Rabbit IgG
  • Preparation
    This antigen of this antibody was synthetic peptide within rat cacng3 aa 200-250.
  • Clonality
    Polyclonal
  • Formulation
    Liquid, 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
  • Storage instructions
    Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
  • Applications

    WB: 1:500

    ICC: 1:50-1:200

    IHC-P: 1:50-1:200

    FC: 1:50-1:100

  • Validations

    Fig1: Western blot analysis of CACNG3 on SH-SY-5Y cell lysates using anti-CACNG3 antibody.; Lane 1: Anti-CACNG3 Antibody (1:500).; Lane 2: Anti-CACNG3 Antibody, pre-incubated with the immunizaiton peptide.

    Fig1: Western blot analysis of CACNG3 on SH-SY-5Y cell lysates using anti-CACNG3 antibody.; Lane 1: Anti-CACNG3 Antibody (1:500).; Lane 2: Anti-CACNG3 Antibody, pre-incubated with the immunizaiton peptide.

    Fig2: ICC staining CACNG3 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig2: ICC staining CACNG3 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig3: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-CACNG3 antibody. Counter stained with hematoxylin.

    Fig3: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-CACNG3 antibody. Counter stained with hematoxylin.

    Fig4: Flow cytometric analysis of SH-SY-5Y cells with CACNG3 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

    Fig4: Flow cytometric analysis of SH-SY-5Y cells with CACNG3 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

  • Background
  • References
    • Spencer KL et al. Dissection of chromosome 16p12 linkage peak suggests a possible role for CACNG3 variants in age-related macular degeneration susceptibility. Invest Ophthalmol Vis Sci. 28;52(3):1748-54 (2011).
    • Everett KV et al. Linkage and association analysis of CACNG3 in childhood absence epilepsy. Eur J Hum Genet. 15(4):463-72 (2007).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"