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Product Name
Anti-C5 (N-term) Rabbit antibody
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Description
C5 (N-term) Rabbit polyclonal antibody
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Tested applications
WB, IHC-P, FC
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Species reactivity
Human
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Isotype
Rabbit IgG
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Preparation
Antigen: This C5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 253-281 amino acids from the N-terminal region of human C5.
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Clonality
Polyclonal
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Formulation
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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Applications
WB::1:1000IHC::1:50~100FCM::1:10~50
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Validations
Flow cytometric analysis of MDA-231 cells using C5 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of C5 Antibody (N-term) (Cat. #169130) in HL-60 cell line lysates (35ug/lane). C5 (arrow) was detected using the purified Pab.(2ug/ml)
Formalin-fixed and paraffin-embedded human breast carcinoma with C5 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical
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Background
Swiss-Prot Acc.P01031.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"