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Product Name
Anti-C4.4A/LYPD3 antibody
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Description
Rabbit monoclonal to C4.4A/LYPD3
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Tested applications
ELISA, WB, FCM, IF, ICC/IF
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Species reactivity
Human C4.4A / LYPD3
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Alternative names
C4.4A antibody; C4.4a antibody; 2310061G07Rik antibody; C4.4a antibody; LYPD3 antibody; UNQ491/PRO1007 antibody; 2310061G07Rik antibody; C4.4A antibody; Lypd3 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human C4.4A / LYPD3 (rh C4.4A / LYPD3; NP_055215.2; Met 1-His 286).
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Clonality
Monoclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 5-10 μg/mL
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human LYPD3. The detection limit for Human LYPD3 is approximately 0.0049 ng/well.
FCM: 0.5-2 μg/Test
ICC/IF: 10-25 μg/mL
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Validations
C4.4A / LYPD3 Antibody, Rabbit MAb, Immunofluorescence
Immunofluorescence staining of Human LYPD3 in PC3 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-Human LYPD3 monoclonal antibody (15 µg/ml) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 594-conjugated goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cell membrane.
C4.4A / LYPD3 Antibody, Rabbit MAb, Western blot
C4.4A / LYPD3 Antibody, Rabbit MAb
Flow cytometric analysis of Human LYPD3 expression on PC-3 cells. Cells were stained with purified anti-Human LYPD3, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
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Background
Ly6 / PLAUR domain-containing protein 3, also known as GPI-anchored metastasis-associated protein C4.4A homolog, Matrigel-induced gene C4 protein, MIG-C4 and LYPD3, is a cell membrane protein which contains two UPAR/Ly6 domains. Human LYPD3 contains two UPAR/Ly6 domains. LYPD3 is expressed in placenta, skin and urothelium. It is found in suprabasal keratinocytes of chronic wounds. Weak expression of LYPD3 is found in esophagus and peripheral blood mononuclear cells. It is found in the majority of primary and metastatic transitional cell carcinomas (TCCs) and as well in breast cancer tissues, but not in adjacent normal tissues. High expression of LYPD3 is found in the tumor component of some noninvasive superficial lesions and in invasive and metastatic urothelial cancers. LYPD3 is up-regulated in migrating keratinocytes during epithelisation of incisional skin wounds. LYPD3 supports cell migration. It may be involved in urothelial cell-matrix interactions. It may also be involved in tumor progression
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References
- Smith B.A., et al., 2001, Cancer Res. 61:1678-85.
- Wuerfel J., et al., 2001, Gene 262:35-41.
- Clark H.F., et al., 2003, Genome Res. 13:2265-70.
- Fletcher G.C., et al., 2003, Br. J. Cancer 88:579-85.
- Hansen L.V., et al., 2004, Biochem. Eng. J. 380:845-57.
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