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Product Name
Anti-C1D antibody
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Description
Rabbit polyclonal to C1D
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Tested applications
ELISA, WB, IHC-P, IP
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Species reactivity
Human C1D
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Alternative names
1110036E10Rik antibody; AI875855 antibody; C1D DNA-binding protein antibody; C1Dnuclearreceptor co-repressor antibody; hC1D antibody; mC1D antibody; MGC12261 antibody; MGC14659 antibody; nuclear DNA binding protein antibody; nuclear DNA-binding protein antibody; nuclear nucleic acid-binding protein C1D antibody; nuclear nucleic acid-binding protein C1D antibody; small unique nuclear co-repressor antibody; small unique nuclearreceptor co-repressor antibody; small unique nuclearreceptorcorepressor antibody; small unique nuclearreceptorcorepressor antibody; sunCoR antibody; SUNCOR antibody; SUN-CoR antibody; SUN-CoR antibody; LRP1 antibody; hC1D antibody; Rrp47 antibody; SUNCOR antibody; SUN-CoR antibody; SUN-CoR antibody; AI875855 antibody; 1110036E10Rik antibody; LRP1 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Human C1D (rh C1D; Q13901; Met 1-Ser 141). C1D specific IgG was purified by Human C1D affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 2-5 μg/mL
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human C1D. The detection limit for Human C1D is approximately 0.00245 ng/well.
IHC-P: 0.1-2 μg/mL
IP: 4-6 μg/mg of lysate
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Validations
C1D Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of human C1D in human kidney with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections). The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).
C1D Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
C1D Antibody, Rabbit PAb, Antigen Affinity Purified
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Background
C1D nuclear receptor corepressor belongs to the C1D family. It is a DNA binding and apoptosis-inducing protein.C1D nuclear receptor corepressorinteracts with TSNAX and DNA-PKcs. It acts as a corepressor for the thyroid hormone receptor. It is thought that C1D nuclear receptor corepressor regulates TRAX/Translin complex formation. It is expressed in kidney, heart, brain, spleen, lung, testis, liver and small intestine. It plays a role in the recruitment of the RNA exosome complex to pre-rRNA to mediate the 3'-5' end processing of the 5.8S rRNA; this function may include MPHOSPH6. It potentiates transcriptional repression by NR1D1 and THRB. C1D nuclear receptor corepressorcan activate PRKDC not only in the presence of linear DNA but also in the presence of supercoiled DNA. It also can induce apoptosis in a p53/TP53 dependent manner.
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References
- Schilders G, et al. (2007) C1D is a major autoantibody target in patients with the polymyositis-scleroderma overlap syndrome. Arthritis Rheum.56(7):2449-54.
- Schilders G, et al. (2007) C1D and hMtr4p associate with the human exosome subunit PM/Scl-100 and are involved in pre-rRNA processing. Nucleic Acids Res. 35(8):2564-72.
- Erdemir T, et al. (2002) DNA damage-dependent interaction of the nuclear matrix protein C1D with Translin-associated factor X (TRAX). J Cell Sci. 115(Pt 1):207-16.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"