-
Product Name
Anti-C17orf75 antibody
- Documents
-
Description
Rabbit polyclonal antibody to C17orf75
-
Tested applications
WB, IHC-P
-
Species reactivity
Human, Mouse, Rat, Dog, Horse, Sheep
-
Alternative names
SRI2 antibody; NJMU-R1 antibody
-
Isotype
Rabbit IgG
-
Preparation
This antigen of this antibody was klh conjugated synthetic peptide derived from human c17orf75 201-300/396
-
Clonality
Polyclonal
-
Formulation
Liquid, 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
-
Storage instructions
Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
-
Applications
WB:1:500-2000
IHC-P:1:400-800
-
Validations
Fig1: Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (C17orf75) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Fig2: Sample:; U2os Cell (Human) Lysate at 30 ug; Primary: Anti- C17orf75 at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 45kD; Observed band size: 45kD
- Background
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"