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Anti-C14orf93 antibody

Cat.#: 176648

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Product Information

  • Product Name
    Anti-C14orf93 antibody
  • Documents
  • Description
    Mouse monoclonal antibody to C14orf93
  • Tested applications
    WB, ICC, IHC-P, FC
  • Species reactivity
    Human
  • Alternative names
    RTFC antibody
  • Isotype
    IgG1
  • Preparation
    This antigen of this antibody was recombinant protein
  • Clonality
    Monoclonal
  • Formulation
    Liquid, 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
  • Storage instructions
    Store at +4℃after thawing. Aliquot store at -20℃or -80℃Avoid repeated freeze / thaw cycles.
  • Applications

    WB: 1:500-1:1,000

    ICC: 1:50-1:200

    IHC-P: 1:50-1:200

    FC: 1:50-1:200

  • Validations

    Fig1: Western blot analysis of C14orf93 on C14orf93-GST recombinant protein lysate using anti-C14orf93 antibody at 1/1,000 dilution.

    Fig1: Western blot analysis of C14orf93 on C14orf93-GST recombinant protein lysate using anti-C14orf93 antibody at 1/1,000 dilution.

    Fig2: ICC staining C14orf93 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig2: ICC staining C14orf93 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig3: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-C14orf93 antibody. Counter stained with hematoxylin.

    Fig3: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-C14orf93 antibody. Counter stained with hematoxylin.

    Fig4: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-C14orf93 antibody. Counter stained with hematoxylin.

    Fig4: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-C14orf93 antibody. Counter stained with hematoxylin.

    Fig5: Immunohistochemical analysis of paraffin-embedded human thyroid tissue using anti-C14orf93 antibody. Counter stained with hematoxylin.

    Fig5: Immunohistochemical analysis of paraffin-embedded human thyroid tissue using anti-C14orf93 antibody. Counter stained with hematoxylin.

    Fig6: Flow cytometric analysis of SH-SY5Y cells with C14orf93 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

    Fig6: Flow cytometric analysis of SH-SY5Y cells with C14orf93 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

  • Background
  • References
    • Yu Y et al. Rtfc (4931414P19Rik) Regulates in vitro Thyroid Differentiation and in vivo Thyroid Function. Sci Rep 7:43396. doi: 10.1038/srep43396 (2017).
    • Liu C et al. C14orf93 (RTFC) is identified as a novel susceptibility gene for familial nonmedullary thyroid cancer. Biochem Biophys Res Commun 482(4):590-596 (2017).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"