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Product Name
Anti-C14orf93 antibody
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Description
Mouse monoclonal antibody to C14orf93
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Tested applications
WB, ICC, IHC-P, FC
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Species reactivity
Human
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Alternative names
RTFC antibody
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Isotype
Mouse IgG1
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Preparation
This antigen of this antibody was recombinant protein
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Clonality
Monoclonal
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Formulation
Liquid, 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
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Storage instructions
Store at +4℃after thawing. Aliquot store at -20℃or -80℃Avoid repeated freeze / thaw cycles.
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Applications
WB: 1:500-1:1,000
ICC: 1:50-1:200
IHC-P: 1:50-1:200
FC: 1:50-1:200
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Validations
Fig1: Western blot analysis of C14orf93 on C14orf93-GST recombinant protein lysate using anti-C14orf93 antibody at 1/1,000 dilution.
Fig2: ICC staining C14orf93 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-C14orf93 antibody. Counter stained with hematoxylin.
Fig4: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-C14orf93 antibody. Counter stained with hematoxylin.
Fig5: Immunohistochemical analysis of paraffin-embedded human thyroid tissue using anti-C14orf93 antibody. Counter stained with hematoxylin.
Fig6: Flow cytometric analysis of SH-SY5Y cells with C14orf93 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
- Background
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References
- Yu Y et al. Rtfc (4931414P19Rik) Regulates in vitro Thyroid Differentiation and in vivo Thyroid Function. Sci Rep 7:43396. doi: 10.1038/srep43396 (2017).
- Liu C et al. C14orf93 (RTFC) is identified as a novel susceptibility gene for familial nonmedullary thyroid cancer. Biochem Biophys Res Commun 482(4):590-596 (2017).
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