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Product Name
Anti-Bruton Tyrosine Kinase / BTK Kinase antibody
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Description
Rabbit polyclonal to Bruton Tyrosine Kinase / BTK Kinase
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Tested applications
WB, IHC-P, IP
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Species reactivity
Human Bruton Tyrosine Kinase / BTK Kinase
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Alternative names
BPK antibody; MGC126261 antibody; MGC126262 antibody; PSCTK1 antibody; RP1-164F3.2 antibody; RP23-91G19.5 antibody; xid antibody; AI528679 antibody; AT antibody; ATK antibody; BPK antibody; XLA antibody; IMD1 antibody; AGMX1 antibody; PSCTK1 antibody; AGMX1 antibody; AI528679 antibody; AT antibody; ATK antibody; BTK antibody; Btk antibody; IMD1 antibody; xid antibody; XLA antibody
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Human Bruton Tyrosine Kinase / BTK Kinase (rh Bruton Tyrosine Kinase / BTK Kinase; NP_000052.1; Met1-Ser659). Bruton Tyrosine Kinase / BTK Kinase specific IgG was purified by Human Bruton Tyrosine Kinase / BTK Kinase affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 5-10 μg/mL
IHC-P: 0.1-2 μg/mL
IP: 1-4 μg/mg of lysate
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Validations
Bruton Tyrosine Kinase / BTK Kinase Antibody, Rabbit PAb, Antigen Affinity Purified, Immunoprecipitation
Bruton Tyrosine Kinase / BTK Kinase Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of human BTK in human tonsil with rabbit polyclonal antibody (0.5 µg/mL, formalin-fixed paraffin embedded sections).
Bruton Tyrosine Kinase / BTK Kinase Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of human BTK in human spleen with rabbit polyclonal antibody (0.5 µg/mL, formalin-fixed paraffin embedded sections).
Bruton Tyrosine Kinase / BTK Kinase Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
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Background
Bruton's tyrosine kinase (or BTK) is a type of kinase protein expressed in B lymphocytes and T cells. BTK contains a PH domain which binds phosphatidylinositol(3,4,5)-trisphosphate (PIP3). After binding to PIP3, BTK is induced to phosphorylate phospholipase C, which in turn hydrolyzes PIP2 into two second messagers, IP3 and DAG, which then modulate the activity of downstream proteins during B-cell signaling. Btk is also found implicated in the primary immunodeficiency disease X-linked agammaglobulinemia(Bruton's agammaglobulinemia). BTK played a key role in B-cell maturation as well as mast cell activation through the high-affinity IgE receptor. Patients with X-linked agammaglobulinemia have normal pre-B cell populations in their bone marrow but these B-cells can not mature and enter the circulation.
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References
- Hashimoto S, et al. (1996) Identification of Bruton's tyrosine kinase (Btk) gene mutations and characterization of the derived proteins in 35 X-linked agammaglobulinemia families: a nationwide study of Btk deficiency in Japan. Blood. 88(2): 561-73.
- Ohta Y, et al. (1994) Genomic organization and structure of Bruton agammaglobulinemia tyrosine kinase: localization of mutations associated with varied clinical presentations and course in X chromosome-linked agammaglobulinemia. PNAS. 91(19): 9062-6.
- Smith C, et al. (1994) Expression of Bruton's agammaglobulinemia tyrosine kinase gene, BTK, is selectively down-regulated in T lymphocytes and plasma cells. The Journal of Immunology. 152(2): 557-65.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"