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Anti-Brk/PTK6 (7D6) Mouse antibody

Cat.#: 168440

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Special Price 441.3 USD

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Product Information

  • Product Name
    Anti-Brk/PTK6 (7D6) Mouse antibody
  • Documents
  • Description
    Brk/PTK6 (7D6) Mouse monoclonal antibody
  • Tested applications
    WB, IP
  • Species reactivity
    Human
  • Isotype
    Mouse IgG2b
  • Preparation
    Antigen: Purified recombinant fragment of human PTK6 expressed in E. Coli.
  • Clonality
    Monoclonal
  • Formulation
    Ascitic fluid containing 0.03% sodium azide.
  • Storage instructions
    Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Applications

    WB: 1/500 - 1/2000

    ELISA: 1/10000

  • Validations

    Western blot analysis using PTK6 mouse mAb against Hela (1), A549 (2) and MCF-7 (3) cell lysate.

    Western blot analysis using PTK6 mouse mAb against Hela (1), A549 (2) and MCF-7 (3) cell lysate.

    Immunoprecipitation analysis of Hela cell lysates using Brk mouse mAb.

    Immunoprecipitation analysis of Hela cell lysates using Brk mouse mAb.

    Western blot detection of Brk in K562,THP-1,MCF7,A549 and Hela cell lysates using Brk mouse mAb (1:1000 diluted).Predicted band size:52KDa.Observed band size:52KDa.

    Western blot detection of Brk in K562,THP-1,MCF7,A549 and Hela cell lysates using Brk mouse mAb (1:1000 diluted).Predicted band size:52KDa.Observed band size:52KDa.

  • Background
    Swiss-Prot Acc.Q13882.PTK6 (protein tyrosine kinase 6, BRK or FLJ42088), with 451-amino acid protein (about 52kDa), encods a cytoplasmic nonreceptor protein kinase which may function as an intracellular signal transducer in epithelial tissues. Its presence in the nucleus appears to be linked to suppression of tumor progression. The encoded protein has been shown to undergo autophosphory-lation. Very high level in colon and high levels in small intestine and prostate, and low levels in some fetal tissues. And Expressed at low level in some breast tumors, but not in normal breast. Also found in melanocytes, but not expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Overexpression of this gene in mammary epithelial cells leads to sensitization of the cells to epidermal growth factor and results in a partially transformed phenotype.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"