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Product Name
Anti-BRAT1 Rabbit antibody
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Description
BRAT1 Rabbit polyclonal antibody
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Tested applications
WB, ICC/IF, FC, IP
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Species reactivity
Human
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant protein of human BRAT1
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Clonality
Polyclonal
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Formulation
Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Storage instructions
Store at -20°C. Stable for 12 months from date of receipt.
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Applications
WB: 1/2000-1/10000
ICC/IF: 1/50
FC: 1/20
IP: 1/20
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Validations
Immunocytochemistry of BRAT1(green) in Hela cells using BRAT1 Rabbit pAb at dilution 1/50, and DAPI(blue)
Western blot detection of BRAT1 in K562 cell lysates using BRAT1 Rabbit pAb(1:1000 diluted).Predicted band size:88kDa.Observed band size:88kDa.
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Background
Swiss-Prot Acc.Q6PJG6.Involved in DNA damage response; activates kinases ATM, SMC1A and PRKDC by modulating their phosphorylation status following ionizing radiation (IR) stress (PubMed:16452482, PubMed:22977523). Plays a role in regulating mitochondrial function and cell proliferation (PubMed:25070371). Required for protein stability of MTOR and MTOR-related proteins, and cell cycle progress by growth factors (PubMed:25657994).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"