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Product Name
Anti-BPHL antibody
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Description
Rabbit polyclonal to BPHL
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Tested applications
ELISA, WB, IHC-P, IP
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Species reactivity
Human BPHL
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Alternative names
MCNAA antibody; BPH-RP antibody; VACVASE antibody; AI115341 antibody; 2010012D11Rik antibody; 5730533B08Rik antibody; 2010012D11Rik antibody; 5730533B08Rik antibody; AI115341 antibody; biphenyl hydrolase-like (serine hydrolase antibody; biphenyl hydrolase-like (serine hydrolase) antibody; biphenyl hydrolase-like protein antibody; biphenyl hydrolase-like protein antibody; biphenyl hydrolase-related protein antibody; Bphl antibody; BPHL antibody; BPH-RP antibody; breast epithelial mucin-associated antigen antibody; breast epithelial mucin-associated antigen) antibody; MCNAA antibody; VACVase antibody; VACVASE antibody; valacyclovir hydrolase antibody; valacyclovir hydrolase antibody; valacyclovirase antibody; valacyclovirase antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Human BPHL (rh BPHL; Q86WA6-1; Ser38-Gln291). BPHL specific IgG was purified by Human BPHL affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 5-10 μg/ml
ELISA:0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human BPHL. The detection limit for Human BPHL is < 0.039 ng/well.
IHC-P: 0.1-2 μg/ml
IP: 1-4 μg/mg of lysate
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Validations
BPHL Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
BPHL Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
BPHL Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
BPHL Antibody, Rabbit PAb, Antigen Affinity Purified, Immunoprecipitation
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Background
BPHL is a member of the serine protease family. BPHL is expressed large quantities in liver and kidney and in minor quantities in heart, intestine and skeletal muscle. BPHL is a specific alpha-amino acid ester hydrolase that prefers small, hydrophobic, and aromatic side chains and does not have a stringent requirement for the leaving group other than preferring a primary alcohol. It catalyzes the hydrolytic activation of amino acid ester prodrugs of nucleoside analogs such as valacyclovir and valganciclovir. BPHL also activates valacyclovir to acyclovir. It may play a role in detoxification processes.
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References
- Lai L. et al., 2008, J Biol Chem. 283 (14): 9318-27.
- Davila S. et al., 2010, Genes Immun. 11 (3): 232-8.
- Hendrickson SL. et al., 2010, PLoS One. 5 (9): e12862.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"