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Product Name
Anti-beta Actin (8F10) Mouse antibody
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Description
beta Actin (8F10) Mouse monoclonal antibody
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Tested applications
WB
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Species reactivity
Human, Mouse, Monkey, Goat, Rat, Hamster, Saccharomyces cerevisiae, Arabidopsis, Pichia pastoris, Rabbit, Zebrafish, Drosophila, Rice, Cucumber, Chlamydomonas Reinhardtii, Fish, Pig, Chicken
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Alternative names
A X actin like protein;ACTB;ACTB_HUMAN;Actin;Actin cytoplasmic 1;Actx;b actin;b-actin;Beta cytoskeletal actin;Beta-actin;cytoplasmic 1;Melanoma X actin;N-terminally processed;PS1TP5 binding protein 1;PS1TP5BP1. antibody
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Isotype
Mouse IgG2b
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Preparation
Antigen: Purified recombinant human beta actin protein fragments expressed in E.coli.
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Clonality
Monoclonal
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Formulation
Purified mouse monoclonal in PB(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/10000;Other 1/5000
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Validations
Western blot detection of beta actin in C6,3T3,COS7,CHO-k1,K562 and Goat muscle cell lysates using beta actin mouse mAb (1:10000 diluted).Predicted band size:45KDa.Observed band size:45KDa.
Western blot detection of beta actin in 15ug Arabidopsis and 30ug Arabidopsis cell lysates using beta actin mouse mAb (1:1000diluted).Predicted band size:45KDa.Observed band size:45KDa.
Western blot detection of beta actin in Rabbit Spleen,Rabbit Colon,Rabbit Skeletal muscle,Rabbit Liver and Rabbit Heart cell lysates using beta actin mouse mAb (1:5000 diluted).Predicted band size:45KDa.Observed band size:45KDa.
Western blot detection of beta actin in chlamydomonas cell lysates using beta actin mouse mAb (1:1000 diluted).Predicted band size:45KDa.Observed band size:45KDa.
Western blot detection of beta actin in 5ug & 10ug S.cerevisiae and 5ug & 10ug P.pastoris cell lysates using beta actin mouse mAb (1:5000 diluted).Predicted band size:45KDa.Observed band size:45KDa.
Western blot detection of beta actin in zebrafish roe cell lysates using beta actin mouse mAb (1:1000-1:3000 diluted).Predicted band size:45KDa.Observed band size:45KDa.
Western blot detection of beta actin in leaf of rice and fruit of cucumber cell lysates using beta actin mouse mAb (1:1000 diluted).Predicted band size:45KDa.Observed band size:45KDa.
Western blot detection of beta actin in 20ug & 40ug Fruit fly (Drosophila melanogaster) cell lysates using beta actin mouse mAb (1:3000 diluted).Predicted band size:45KDa.Observed band size:45KDa.
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Background
Swiss-Prot Acc.P60709.Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
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