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Product Name
Anti-Autotaxin/ENPP2 antibody
- Documents
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Description
Rabbit polyclonal to Autotaxin/ENPP2
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Tested applications
ELISA, IHC-P
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Species reactivity
Cynomolgus Autotaxin / ENPP2
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Alternative names
ATX antibody; ATX-X antibody; Enpp2 antibody; NPP2 antibody; Npps2 antibody; PD-Ialpha antibody; PD-IALPHA antibody; Pdnp2 antibody; PDNP2 antibody; ATX antibody; AUTOTAXIN antibody; autotaxin antibody; ENPP2 antibody; FLJ26803 antibody; LysoPLD antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Cynomolgus Autotaxin / ENPP2 (rh Autotaxin / ENPP2; XP_005564040.1; Asp49-Ile863). Autotaxin / ENPP2 specific IgG was purified by Cynomolgus Autotaxin / ENPP2 affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
ELISA:0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Cynomolgus Autotaxin / ENPP2. The detection limit for Cynomolgus Autotaxin / ENPP2 is < 0.039 ng/well.
IHC-P: 0.1-2 μg/ml
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Validations
Autotaxin / ENPP2 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Autotaxin / ENPP2 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Autotaxin / ENPP2 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
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Background
ENPP2 (Ectonucleotide pyrophosphatase/phosphodiesterase family member 2), also referred as Autotaxin, is a secreted enzyme encoded by the ENPP2 gene. This gene product stimulates the motility of tumor cells, has angiogenic properties, and its expression is upregulated in several kinds of carcinomas. The Autotaxin protein is important for generating the lipid signaling molecule lysophosphatidic acid (LPA), which is a potent mitogen, which facilitates cell proliferation and migration, neurite retraction, platelet aggregation, smooth muscle contraction, actin stress formation and cytokine and chemokine secretion. ATX has been found to catalyze the formation of cyclic phosphatidic acid (cPA), which have antitumor role by antimitogenic regulation of cell cycle, inhibition of cancer invasion and metastasis. LPA receptors and ATX are upregulated in numerous cancer cell types and show expression patterns that correlate with tumor cell invasiveness. Thus, Autotaxin has recently emerged as an attractive target for the development of anti-cancer chemotherapeutics. In addition, Serum ATX activity was found to be enhanced in relation to hepatic fibrosis in chronic liver disease due to hepatitis virus C infection.
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References
- Tania M, et al. (2010) Autotaxin: a protein with two faces. Biochem Biophys Res Commun. 401(4): 493-7.
- Ikeda H, et al. (2009) Significance of serum autotaxin activity in gastrointestinal disease. Rinsho Byori. 57(5): 445-9.
- Parrill AL, et al. (2008) Autotaxin inhibition: challenges and progress toward novel anti-cancer agents. Anticancer Agents Med Chem. 8(8): 917-23.
- Pradere J.P, et al. (2007) Secretion and lysophospholipase D activity of autotaxin by adipocytes are controlled by N-glycosylation and signal peptidase. Biochim Biophys Acta. 1771: 93-102.
- Boucher J, et al. (2005) Potential involvement of adipocyte insulin resistance in obesity-associated up-regulation of adipocyte lysophospholipase D/autotaxin expression. Diabetologia. 48: 569-77.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"