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Anti-AUP1 antibody

Cat.#: 175075

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Special Price 241.9 USD

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Product Information

  • Product Name
    Anti-AUP1 antibody
  • Documents
  • Description
    Mouse monoclonal antibody to AUP1
  • Tested applications
    WB
  • Species reactivity
    Human, Mouse
  • Isotype
    Mouse IgG2b
  • Preparation
    This antigen of this antibody was purified recombinant fragment of human aup1 (aa: 229-410) expressed in e. coli.
  • Clonality
    Monoclonal
  • Formulation
    Liquid, 1*PBS with 0.05% sodium azide.
  • Storage instructions
    Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
  • Applications

    WB: 1:500-1:2,000

  • Validations

    Fig1: Western blot analysis of AUP1 against human AUP1 (AA: 229-410) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.

    Fig1: Western blot analysis of AUP1 against human AUP1 (AA: 229-410) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.

    Fig2: Western blot analysis of 175075# against HEK293 (1) and AUP1 (AA: 229-410)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.

    Fig2: Western blot analysis of 175075# against HEK293 (1) and AUP1 (AA: 229-410)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.

    Fig3: Western blot analysis of 175075# against A431 (1), NIH/3T3 (2), Hela (3), SW480 (4), CHO3D10 (5), A549 (6), and SPC-A-1 (7) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.

    Fig3: Western blot analysis of 175075# against A431 (1), NIH/3T3 (2), Hela (3), SW480 (4), CHO3D10 (5), A549 (6), and SPC-A-1 (7) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.

  • Background
    Q9Y679
  • References
    • J Lipid Res. 2013 Feb;54(2):503-13.
    • J Biol Chem. 2011 Feb 18;286(7):5599-606.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"