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Anti-ATRAID antibody

Cat.#: 175130

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Product Information

  • Product Name
    Anti-ATRAID antibody
  • Documents
  • Description
    Rabbit polyclonal antibody to ATRAID
  • Tested applications
    WB, ICC, IHC-P, FC
  • Species reactivity
    Human, Mouse, Rat
  • Alternative names
    p18 antibody; APR3 antibody; APR-3 antibody; APR--3 antibody; PRO240 antibody; C2orf28 antibody; HSPC013 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antigen of this antibody was synthetic peptide corresponding to mouse apr3 c terminal.
  • Clonality
    Polyclonal
  • Formulation
    Liquid, 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
  • Storage instructions
    Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
  • Applications

    WB: 1:500-2,000

    ICC: 1:50-1:200

    IHC-P: 1:50-1:200

    FC: 1:50-1:100

  • Validations

    Fig1: Western blot analysis of APR3 on human skin tissue lysates using anti-APR3 antibody.; Lane 1: Anti-APR3 antibody (1/500).; Lane 2: Anti-APR3 antibody, pre-incubated with the immunizaiton peptide.

    Fig1: Western blot analysis of APR3 on human skin tissue lysates using anti-APR3 antibody.; Lane 1: Anti-APR3 antibody (1/500).; Lane 2: Anti-APR3 antibody, pre-incubated with the immunizaiton peptide.

    Fig2: ICC staining APR3 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig2: ICC staining APR3 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig3: ICC staining APR3 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig3: ICC staining APR3 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig4: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-APR3 antibody. Counter stained with hematoxylin.

    Fig4: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-APR3 antibody. Counter stained with hematoxylin.

    Fig5: Flow cytometric analysis of MCF-7 cells with APR3 antibody at 1/100 dilution (Pink purple) compared with an unlabelled control (cells without incubation with primary antibody; Yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

    Fig5: Flow cytometric analysis of MCF-7 cells with APR3 antibody at 1/100 dilution (Pink purple) compared with an unlabelled control (cells without incubation with primary antibody; Yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

  • Background
    Q6UW56
  • References
    • Zou X et al. NELL-1 binds to APR3 affecting human osteoblast proliferation and differentiation. FEBS Lett. 585:2410-2418 (2011).
    • Yu F et al. Apoptosis related protein 3, an ATRA-upregulated membrane protein arrests the cell cycle at G1/S phase by decreasing the expression of cyclin D1. Biochem. Biophys. Res. Commun. 358:1041-1046 (2007).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"