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Product Name
Anti-ATP citrate Lyase (3D9) Mouse antibody
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Description
ATP citrate Lyase (3D9) Mouse monoclonal antibody
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Tested applications
WB, ICC/IF, FC
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Species reactivity
Human, Mouse, Monkey
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Alternative names
ACL;Acly;ACLY_HUMAN;ATP citrate (pro-S) lyase;ATP citrate lyase;ATP citrate synthase; ATP-citrate (pro-S-)-lyase;ATP-citrate synthase;ATPcitrate synthase;ATPCL;Citrate cleavage enzyme;CLATP;EC 2.3.3.8;OTTHUMP00000164773. antibody
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Isotype
Mouse IgG2a
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Preparation
Antigen: Purified recombinant human ATP-Citrate Lyase protein fragments expressed in E.coli.
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Clonality
Monoclonal
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Formulation
Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4 150 mM NaCl) with 0.02% sodium azide 50% glycerol
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/1000
;ICC: 1/150
;FC: 1/100
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Validations
Flow Cytometry analysis of HeLa cells stained with ATP-Citrate Lyase (red, 1/100 dilution), followed by FITC-conjugated goat anti-mouse IgG. Black line histogram represents the isotype control, normal mouse IgG
Western blot detection of ATP-Citrate Lyase in 3T3,K562,COS7 and Hela cell lysates using ATP-Citrate Lyase mouse mAb (1:1000 diluted).Predicted band size: 120KDa.Observed band size: 120KDa.
Immunocytochemistry of HeLa cells using anti-ATP-Citrate Lyase (C-terminus) mouse mAb diluted 1:150.
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Background
Swiss-Prot Acc.P53396.ATP citrate-lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. Has a central role in de novo lipid synthesis. In nervous tissue it may be involved in the biosynthesis of acetylcholine.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"