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Product Name
Anti-ATG7 Rabbit antibody
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Description
ATG7 Rabbit polyclonal antibody
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Tested applications
WB, IHC-P, ICC/IF
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Species reactivity
Human, Mouse, Rat
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant protein of human ATG7 (NP_001129503.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20°C . Avoid freeze / thaw cycles.
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Applications
WB: 1/500 - 1/2000
;IHC: 1/50 - 1/200
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Validations
Immunohistochemistry - ATG7 Polyclonal Antibody. Immunohistochemistry of paraffin-embedded human tonsil using ATG7 antibody at dilution of 1:100 (40x lens).
Western blot - ATG7 Polyclonal Antibody.Western blot analysis of extracts of various cell lines, using ATG7 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Exposure time: 60s.
Immunohistochemistry - ATG7 Polyclonal Antibody. Immunohistochemistry of paraffin-embedded rat brain using ATG7 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - ATG7 Polyclonal Antibody. Immunohistochemistry of paraffin-embedded mouse brain using ATG7 antibody at dilution of 1:100 (40x lens).
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Background
Swiss-Prot Acc.O95352.
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References
- Inhibiting autophagy increases epirubicin's cytotoxicity in breast cancer cells
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"