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Product Name
Anti-ATG4C Rabbit antibody
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Description
ATG4C Rabbit polyclonal antibody
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Tested applications
WB, IHC-P, FC
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Species reactivity
Human
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Alternative names
APG4C; AUTL1; AUTL3; APG4-C antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant protein of human ATG4C
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Clonality
Polyclonal
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Formulation
Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Storage instructions
Store at -20°C. Stable for 12 months from date of receipt.
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Applications
WB: 1/1000
IHC: 1/50
FC: 1/20
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Validations
Western blot detection of ATG4C in K562,Hela cell lysates using ATG4C Rabbit pAb(1:1000 diluted).Predicted band size:53kDa.Observed band size:53kDa.
Immunohistochemistry of ATG4C in paraffin-embedded Human lung cancer tissue using ATG4C Rabbit pAb at dilution 1/50
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Background
Swiss-Prot Acc.Q96DT6.Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Is not essential for autophagy development under normal conditions but is required for a proper autophagic response under stressful conditions such as prolonged starvation . Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3 and GABARAPL2, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"