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Product Name
Anti-ATG12 Rabbit antibody
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Description
ATG12 Rabbit polyclonal antibody
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Tested applications
WB, ICC/IF
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Species reactivity
Human
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Alternative names
APG12; FBR93; APG12L; HAPG12 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide of human Apg12
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Clonality
Polyclonal
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Formulation
Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Storage instructions
Store at -20°C. Stable for 12 months from date of receipt.
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Applications
WB: 1/1000-1/5000
ICC/IF: 1/50-1/200
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Validations
Western blot detection of Apg12 in K562,3T3,Hela cell lysates using Apg12 Rabbit pAb(1:1000 diluted).Predicted band size:15kDa.Observed band size:55kDa.
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Background
Swiss-Prot Acc.O94817.Ubiquitin-like protein involved in autophagy vesicles formation. Conjugation with ATG5 through a ubiquitin-like conjugating system involving also ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"