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Product Name
Anti-ATG10 Rabbit antibody
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Description
ATG10 Rabbit monoclonal antibody
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Tested applications
WB, IP
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Species reactivity
Human
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide of human ATG10
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Clonality
Monoclonal
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Formulation
50nM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/1000
IP: 1/20
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Validations
Western blot detection of ATG10 in Hela,A549,U251 cell lysates using ATG10 Rabbit mAb(1:1000 diluted).Predicted band size:25kDa.Observed band size:25kDa.
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Background
Autophagy is a process for the bulk degradation of cytosolic compartments by lysosomes. ATG10 is an E2-like enzyme involved in 2 ubiquitin-like modifications essential for autophagosome formation: ATG12 (MIM 609608)-ATG5 (MIM 604261) conjugation and modification of a soluble form of MAP-LC3 (MAP1LC3A; MIM 601242), a homolog of yeast Apg8, to a membrane-bound form (Nemoto et al., 2003 [PubMed 12890687]).[supplied by OMIM, Mar 2008]
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"