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Product Name
Anti-ATF2 antibody
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Description
Rabbit polyclonal to ATF2
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Tested applications
IF, ICC/IF
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Species reactivity
Human ATF2
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Alternative names
HB16 antibody; CREB2 antibody; TREB7 antibody; CREB-2 antibody; CRE-BP1 antibody; mXBP antibody; Atf-2 antibody; Creb2 antibody; CRE-BP antibody; D18875 antibody; D130078H02Rik antibody; Tg(Gzma-Klra1)7Wum antibody; Atf-2 antibody; CREB2 antibody; Creb2 antibody; CRE-BP antibody; CRE-BP1 antibody; D130078H02Rik antibody; D18875 antibody; HB16 antibody; MGC105211 antibody; MGC105222 antibody; MGC111558 antibody; RP23-290B1.2 antibody; Tg(Gzma-Klra antibody; TREB7 antibody
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human ATF2, and purified by antigen affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
ICC/IF: 0.5-1.5 μg/mL
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Validations
ATF2 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunofluorescence
Immunofluorescence staining of ATF2 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human ATF2 polyclonal antibody (1 µg/ml) at 4℃ overnight. Then cells were st
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Background
Activating transcription factor 2, also known as ATF2, is a member of the leucine zipper family of DNA-binding proteins that binds to the cAMP response element. Its activity is enhanced after phosphorylation by stress-activated protein kinases such as c-Jun N-terminal kinase and p38. ATF2 has been found to be a target of the JNK signal transduction pathway and mediate adenovirus E1A-inducible transcriptional activation. ATF2 is also been reported playing roles in TGF-β signaling pathway. It has been shown that the transcription factor ATF2 is bound by a hetero-oligomer of Smad3 and Smad4 upon TGF-β stimulation. Studies indicate that ATF-2 plays a central role in TGF-β signaling by acting as a common nuclear target of both Smad and TAK1 pathways.
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References
- Livingstone C, et al. (1995) ATF-2 contains a phosphorylation-dependent transcriptional activation domain. EMBO J. 14 (8): 1785-97.
- Gupta S, et al. (1995) Transcription factor ATF2 regulation by the JNK signal transduction pathway. Science . 267 (5196): 389-93.
- Sano YJ, et al. (1999) ATF-2 Is a Common Nuclear Target of Smad and TAK1 Pathways in Transforming Growth Factor-_ Signaling. The Journal of Biological Chemistry. 274: 8949-57.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"