Anti-ASIC1 antibody

Cat.#: 175127

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Product Information

  • Product Name
    Anti-ASIC1 antibody
  • Documents
  • Description
    Rabbit polyclonal antibody to ASIC1
  • Tested applications
    WB, IHC-P, FC
  • Species reactivity
    Human, Mouse, Rat
  • Alternative names
    ASIC antibody; ACCN2 antibody; BNaC2 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antigen of this antibody was synthetic peptide within human accn2 aa 460-500.
  • Clonality
    Polyclonal
  • Formulation
    Liquid, 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
  • Storage instructions
    Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
  • Applications

    WB: 1:500-1:2,000

    IHC-P: 1:50-1:200

    FC: 1:50-1:100

  • Validations

    Fig1: Western blot analysis of ACCN2 on SH-SY-5Y cell lysate using anti-ACCN2 antibody at 1/2,000 dilution.

    Fig1: Western blot analysis of ACCN2 on SH-SY-5Y cell lysate using anti-ACCN2 antibody at 1/2,000 dilution.

    Fig2: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-ACCN2 antibody. Counter stained with hematoxylin.

    Fig2: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-ACCN2 antibody. Counter stained with hematoxylin.

    Fig3: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-ACCN2 antibody. Counter stained with hematoxylin.

    Fig3: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-ACCN2 antibody. Counter stained with hematoxylin.

    Fig4: Flow cytometric analysis of SH-SY-5Y cells with ACCN2 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

    Fig4: Flow cytometric analysis of SH-SY-5Y cells with ACCN2 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

  • Background
  • References
    • Dawson R J et al. Structure of the acid-sensing ion channel 1 in complex with the gating modifier Psalmotoxin 1. Nat Commun 3:936-943 (2012).
    • Sherwood T et al. Identification of protein domains that control proton and calcium sensitivity of ASIC1a. J Biol Chem 284:27899-27907 (2009).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"