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Product Name
Anti-APEX1/AP/APEx/Ref-1 antibody
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Description
Rabbit polyclonal to APEX1/AP/APEx/Ref-1
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Tested applications
WB, IHC-P, IF, IP, ICC/IF
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Species reactivity
Human APEX1 / AP / APEx / Ref-1
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Alternative names
APE antibody; APX antibody; APE1 antibody; APEN antibody; APEX antibody; HAP1 antibody; REF1 antibody; APE antibody; Apex antibody; HAP1 antibody; Ref-1 antibody; APE antibody; APE antibody; APE1 antibody; APEN antibody; APEX antibody; Apex antibody; APX antibody; HAP1 antibody; HAP1 antibody; Ref-1 antibody; REF1 antibody
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human APEX1 / AP / APEx / Ref-1, and purified by antigen affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB:2-10 μg/ml
IHC-P: 0.1-2 μg/mL
ICC/IF: 0.5-1.5μg/mL
IP: 0.5-2 uL/mg of lysate
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Validations
APEX1 / AP / APEx / Ref-1 Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
APEX1 / AP / APEx / Ref-1 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of human APEX1 in human breast with rabbit polyclonal antibody (0.5 µg/mL, formalin-fixed paraffin embedded sections).
APEX1 / AP / APEx / Ref-1 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of human APEX1 in human placenta with rabbit polyclonal antibody (0.5 µg/mL, formalin-fixed paraffin embedded sections).
APEX1 / AP / APEx / Ref-1 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunofluorescence
Immunofluorescence staining of APEX1 in HeLa cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-human APEX1 polyclonal antibody (1 µg/ml) at 4℃ overnight. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody (green)Positive staining was localized to nucleus.
APEX1 / AP / APEx / Ref-1 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunoprecipitation
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Background
The enzyme is known to be a redox factor (Ref-1) stimulating DNA binding activity of AP-1 binding proteins such as Fos and Jun as well as a multifunctional DNA repair enzyme having 5' AP endonuclease, DNA 3' repair diesterase, 3'-5' exonuclease and DNA 3'-phosphatase activities.Although Apex mRNA was expressed ubiquitously, the levels varied significantly, suggesting organ- or tissue-specific expression of the Apex gene. The highest level was observed in the testis, relatively high levels in the thymus, spleen, kidney and brain, and the lowest level in the liver in rats. However, the present results suggested that APEX/Ref-1 gene product can interact with AP-1 binding proteins in brain, especially in the hippocampal formation, to regulate some brain functions by redox-activation.
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References
- Ono Y, et al. (1995) Developmental expression of APEX nuclease, a multifunctional DNA repair enzyme, in mouse brains. Brain Res Dev Brain Res.86 (1-2): 1-6.
- Tan Y, et al. (1996) cDNA cloning of rat major AP endonuclease (APEX nuclease) and analyses of its mRNA expression in rat tissues. Acta Med Okayama. 50 (1): 53-60.
- Yao M, et al. (1999) Genomic structure of the rat major AP endonuclease gene (Apex) with an adjacent putative O-sialoglycoprotease gene (Prsmg1/Gcpl1) and a processed Apex pseudogene (Apexp1). Acta Med Okayama. 53 (6): 245-52.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"