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Product Name
Anti-Anti-ACTR2 (C-term) Rabbit antibody
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Description
Anti-ACTR2 (C-term) Rabbit polyclonal antibody
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Tested applications
WB, IHC-P, FC
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Species reactivity
Human
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Isotype
Rabbit IgG
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Preparation
Antigen: This ACTR2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 323-352 amino acids from the C-terminal region of human ACTR2.
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Clonality
Polyclonal
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Formulation
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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Applications
WB::1:1000IHC::1:50~100FCM::1:10~50
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Validations
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with ACTR2 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry;
All lanes: Anti-ACTR2 Antibody (C-term) at 1:1000 dilutionnLane 1: Hela whole cell lysatenLane 2: MCF-7 whole cell lysatennLysates/proteins at 20 u03bcg per lane. nnSecondarynGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. nnPredicted band size: 45 kDannBlocking/Dilution buffer: 5% NFDM/TBST.
ACTR2 Antibody (C-term) (Cat. #AP6533b) flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Background
Swiss-Prot Acc.P61160.
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